ArticleScientific reports2019
Extracellular endonucleases in the midgut of Myzus persicae may limit the efficacy of orally delivered RNAi.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- LDH encapsulation and MpdsRNase disruption boost RNAi efficacy in Myzus persicae, with mRNA-miRNA profiles unraveling lethality by tandem dsRNA targeting MpEcR and MpdsRNase.Pest management science · 2026Article
- Nanoparticle-enhanced multi-target RNAi in Myzus persicae: Molecular mechanisms, cross-kingdom sRNA transfer, and efficacy for sustainable pest management.Pest management science · 2026Article
- Delivery, Persistence, and Functional Response of dsRNA in Acyrthosiphon pisum: Progress and Remaining Barriers.Archives of insect biochemistry and physiology · 2026Article
- Overview of polymeric polyplexes for dsRNA delivery in insects: complexation, stability, and design considerations.Frontiers in insect science · 2026Review
- Article
- Ecological Mercenaries: Why Aphids Remain Premier Models for the Study of Ecological Symbiosis.Insects · 2025Review
- Transgenic expression of mAChR-C dsRNA in maize confers efficient locust control.Plant communications · 2025Article
- Targeting vacuolar ATPase in tobacco whitefly,Journal of biosciences · 2025Review
- Article
- Facultative symbionts are potential agents of symbiont-mediated RNAi in aphids.Frontiers in microbiology · 2022Review
- Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide.Frontiers in zoology · 2021Review
- Transgenic Expression of dsRNA Targeting thePlants (Basel, Switzerland) · 2021Article
- Effectiveness of orally-delivered double-stranded RNA on gene silencing in the stinkbug Plautia stali.PloS one · 2021Article
- RNAi-Based Functional Genomics in Hemiptera.Insects · 2020Review
- Advances in exogenous RNA delivery techniques for RNAi-mediated pest control.Molecular biology reports · 2020Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myzus persicae is a major pest of many crops including canola and Brassica vegetables, partly because it vectors plant viruses. Previously it has been reported that double-stranded RNA delivered to aphids by injection, artificial diet or transgenic plants has knocked down target genes and caused phenotypic effects. While these studies suggest that RNA interference (RNAi) might be used to suppress aphid populations, none have shown effects sufficient for field control. The current study analyses the efficacy of dsRNA directed against previously reported gene-targets on Green peach aphid (Myzus persicae) strains. No silencing effect was observed when dsRNA was delivered in artificial diet with or without transfection reagents. dsRNA produced in planta also failed to induce significant RNAi in M. persicae. Transcriptome analyses of the midgut suggested other potential targets including the Ferritin heavy chain transcripts, but they also could not be knocked down with dsRNA. Here we show that dsRNA is rapidly degraded by midgut secretions of Myzus persicae. Analysis of the transcriptome of the M. persicae midgut revealed that an ortholog of RNases from other insects was abundant.
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