ArticleParasites & vectors2019
Detecting wMel Wolbachia in field-collected Aedes aegypti mosquitoes using loop-mediated isothermal amplification (LAMP).
Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Locally Acquired Dengue in Townsville, Australia, 2024-2025: An Outbreak Report in a Non-Endemic Region withTropical medicine and infectious disease · 2026Article
- Successful introgression of wMel Wolbachia into Aedes aegypti populations in Fiji, Vanuatu and Kiribati.PLoS neglected tropical diseases · 2024Article
- Measuring Host Fitness Effects and Transmission of Wolbachia Strains in Aedes aegypti Mosquitoes.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Improved visual detection of DNA amplification using pyridylazophenol metal sensing dyes.Communications biology · 2022Article
- Simple, sensitive, and cost-effective detection of wAlbB Wolbachia in Aedes mosquitoes, using loop mediated isothermal amplification combined with the electrochemical biosensing method.PLoS neglected tropical diseases · 2022Article
- A wMel Wolbachia variant in Aedes aegypti from field-collected Drosophila melanogaster with increased phenotypic stability under heat stress.Environmental microbiology · 2022Article
- Wolbachia detection in Aedes aegypti using MALDI-TOF MS coupled to artificial intelligence.Scientific reports · 2021Article
- Initial Evaluation of a Mobile SARS-CoV-2 RT-LAMP Testing Strategy.Journal of biomolecular techniques : JBT · 2021Article
- Corona Detective: a simple, scalable, and robust SARS-CoV-2 detection method based on reverse transcription loop-mediated isothermal amplification.Journal of biomolecular techniques : JBT · 2021Article
- Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications.Journal of biomolecular techniques : JBT · 2021Review
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- Initial evaluation of a mobile SARS-CoV-2 RT-LAMP testing strategy.medRxiv : the preprint server for health sciences · 2021Article
- Large-Scale Deployment and Establishment ofFrontiers in microbiology · 2021Article
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- A LAMP-based colorimetric assay to expedite field surveillance of the invasive mosquito species Aedes aegypti and Aedes albopictus.PLoS neglected tropical diseases · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe World Mosquito Program uses Wolbachia pipientis for the biocontrol of arboviruses transmitted by Aedes aegypti mosquitoes. Diagnostic testing for Wolbachia in laboratory colonies and in field-caught mosquito populations has typically employed PCR. New, simpler methods to diagnose Wolbachia infection in mosquitoes are required for large-scale operational use.
methodsField-collected Ae. aegypti mosquitoes from North Queensland were tested using primers designed to detect the Wolbachia wsp gene, specific to the strain wMel. The results were analysed by colour change in the reaction mix. Furthermore, to confirm the efficiency of the LAMP assay, the results were compared to the gold-standard qPCR test.
resultsA novel loop-mediated isothermal amplification (LAMP) colorimetric test for the wMel strain of Wolbachia was designed, developed and validated for use in a high-throughput setting. Against the standard qPCR test, the analytical sensitivity, specificity and diagnostic metrics were: sensitivity (99.6%), specificity (92.2%), positive predictive value (97.08%) and negative predictive value (99.30%).
conclusionsWe describe an alternative, novel and high-throughput method for diagnosing wMel Wolbachia infections in mosquitoes. This assay should support Wolbachia surveillance in both laboratory and field populations of Ae. aegypti.
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