Evidence map›Paper›PMID 31414341›Full record

ArticleJournal of physiology and biochemistry2019

Transcriptome analysis reveals the molecular mechanisms of combined gamma-tocotrienol and hydroxychavicol in preventing the proliferation of 1321N1, SW1783, and LN18 glioma cancer cells.

Amirah Abdul Rahman, Norfilza Mohd Mokhtar, Roslan Harun, Rahman Jamal, Wan Zurinah Wan Ngah

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In one paragraph

Article in Journal of physiology and biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Amirah Abdul RahmanDepartment Biochemistry & Molecular Medicine, Faculty of Medicine, Universiti Teknologi MARA, Cawangan Selangor, Kampus Sungai Buloh, Jalan Hospital, 47000, Sungai Buloh, Selangor, Malaysia.ORCID http://orcid.org/0000-0002-3566-1787
Norfilza Mohd MokhtarDepartment of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Ya'acob, Bandar Tun Razak, Cheras, 56000, Kuala Lumpur, Malaysia. norfilza@ppukm.ukm.edu.my.
Roslan HarunKPJ Ampang Putri Hospital, 1 Jalan Mamanda 9, Taman Dato Ahmad Razali, 68000, Ampang, Selangor, Malaysia.
Rahman JamalUKM Medical Molecular Biology Institute (UMBI), UKM Medical Center, Jalan Ya'acob, Bandar Tun Razak, Cheras, 56000, Kuala Lumpur, Malaysia.
Wan Zurinah Wan NgahDepartment of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Ya'acob, Bandar Tun Razak, Cheras, 56000, Kuala Lumpur, Malaysia.
University Kebangsaan Malaysia Medical Centre · MYKPJ Ampang Puteri Specialist Hospital · MYNational University of Malaysia · MY

Funding

Ministry of Higher Education, Malaysia. 10-64-01-005
6 · The paper itself

Abstract

Gamma-tocotrienol (GTT) and hydroxychavicol (HC) exhibit anticancer activity in glioma cancer cells, where the combination of GTT + HC was shown to be more effective than single agent. The aim of this study was to determine the effect of GTT + HC by measuring the cell cycle progression, migration, invasion, and colony formation of glioma cancer cells and elucidating the changes in gene expression mitigated by GTT + HC that are critical to the chemoprevention of glioma cell lines 1321N1 (grade II), SW1783 (grade III), and LN18 (grade IV) using high-throughput RNA sequencing (RNA-seq). Results of gene expression levels and alternative splicing transcripts were validated by qPCR. Exposure of glioma cancer cells to GTT + HC for 24 h promotes cell cycle arrest at G2M and S phases and inhibits cell migration, invasion, and colony formation of glioma cancer cells. The differential gene expression induced by GTT + HC clustered into response to endoplasmic reticulum (ER) stress, cell cycle regulations, apoptosis, cell migration/invasion, cell growth, and DNA repair. Subnetwork analysis of genes altered by GTT + HC revealed central genes, ATF4 and XBP1. The modulation of EIF2AK3, EDN1, and FOXM1 were unique to 1321N1, while CSF1, KLF4, and FGF2 were unique to SW1783. PLK2 and EIF3A gene expressions were only altered in LN18. Moreover, GTT + HC treatment dynamically altered transcripts and alternative splicing expression. GTT + HC showed therapeutic potential against glioma cancer as evident by the inhibition of cell cycle progression, migration, invasion, and colony formation of glioma cancer cells, as well as the changes in gene expression profiles with key targets in ER unfolded protein response pathway, apoptosis, cell cycle, and migration/invasion.

Indexed as

Antineoplastic AgentsApoptosisBrain NeoplasmsCell Cycle CheckpointsCell Line, TumorCell MovementCell ProliferationChromansEugenolGene Expression Regulation, NeoplasticGliomaHumansKruppel-Like Factor 4Vitamin E2-hydroxychavicolAntineoplastic AgentsChromansEugenolKLF4 protein, humanKruppel-Like Factor 4plastochromanol 8Vitamin EChemopreventionGamma-tocotrienolGene expressionHydroxychavicolRNA sequencingSynergistic effects

Identifiers

PMID31414341
OpenAlexW2967600352

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.