ArticleJournal of assisted reproduction and genetics2019
Gene expression analysis of ovine prepubertal testicular tissue vitrified with a novel cryodevice (E.Vit).
Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Intraepididymal platelet-rich plasma improves semen cryoresistance via antioxidant, lipid and molecular modulation during the non-breeding season in rams.Veterinary research communications · 2026Article
- Slow freezing and 3DAnimal reproduction · 2026Article
- Cryopreservation and culture strategies for testicular tissue and cells in small and large animals.Frontiers in veterinary science · 2025Review
- Testicular Tissue Vitrification: a Promising Strategy for Male Fertility Preservation.Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- Technologies for Vitrification Based Cryopreservation.Bioengineering (Basel, Switzerland) · 2023Review
- Current scenario and challenges ahead in application of spermatogonial stem cell technology in livestock.Journal of assisted reproduction and genetics · 2021Review
- Molecular and Histological Evaluation of Sheep Ovarian Tissue Subjected to Lyophilization.Animals : an open access journal from MDPI · 2021Article
- Strategies for cryopreservation of testicular cells and tissues in cancer and genetic diseases.Cell and tissue research · 2021Review
- HighJournal of animal science and biotechnology · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
purposeTesticular tissue cryopreservation prior to gonadotoxic therapies is a method to preserve fertility in children. However, the technique still requires development, especially when the tissue is immature and rather susceptible to stress derived from in vitro manipulation. This study aimed to investigate the effects of vitrification with a new cryodevice (E.Vit) on cell membrane integrity and gene expression of prepubertal testicular tissue in the ovine model.
methodsPieces of immature testicular tissue (1 mm
resultsPost-warming trypan blue staining showed the survival of most cells, although membrane integrity immediately after warming (66.00% ± 4.73) or after 2 h IVC (59.67% ± 4.18) was significantly lower than controls (C0h 89.67% ± 1.45). Extended post-warming IVC (24 h) caused an additional decrease to 31% ± 3.46 (P < 0.05). Germ- and somatic-cell specific markers showed the survival of both cell types after cryopreservation and IVC. All genes were affected by cryopreservation and/or IVC, and moderate stress conditions were indicated by transcriptional stress response.
conclusionsVitrification with the cryodevice E.Vit is a promising strategy to cryopreserve prepubertal testicular tissue.
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Registered trials
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