ArticleThe Journal of cell biology1988
The role of kinesin and other soluble factors in organelle movement along microtubules.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 178 citations in OpenAlex.
- Inhibition of glycogen synthase kinase 3 by lithium, a mechanism in search of specificity.Frontiers in molecular neuroscience · 2022Review
- AMPK Preferentially Depresses Retrograde Transport of Axonal Mitochondria during Localized Nutrient Deprivation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.Scientific reports · 2019Article
- Membrane mediated motor kinetics in microtubule gliding assays.Scientific reports · 2019Article
- Article
- Acrylamide Retards the Slow Axonal Transport of Neurofilaments in Rat Cultured Dorsal Root Ganglia Neurons and the Corresponding Mechanisms.Neurochemical research · 2016Article
- Single-molecule fluorescence and in vivo optical traps: how multiple dyneins and kinesins interact.Chemical reviews · 2014Review
- Reconstituting the motility of isolated intracellular cargoes.Methods in enzymology · 2014Article
- Quantitative measurements and modeling of cargo-motor interactions during fast transport in the living axon.Physical biology · 2012Article
- Bidirectional power stroke by ncd kinesin.Biophysical journal · 2010Article
- The biochemistry, ultrastructure, and subunit assembly mechanism of AMPA receptors.Molecular neurobiology · 2010Review
- Spectrin mutations that cause spinocerebellar ataxia type 5 impair axonal transport and induce neurodegeneration in Drosophila.The Journal of cell biology · 2010Article
- No conventional function for the conventional kinesin?Traffic (Copenhagen, Denmark) · 2008Article
- Huntingtin phosphorylation acts as a molecular switch for anterograde/retrograde transport in neurons.The EMBO journal · 2008Article
- Direct observation of regulated ribonucleoprotein transport across the nurse cell/oocyte boundary.Molecular biology of the cell · 2007Article
- Dynactin is required for bidirectional organelle transport.The Journal of cell biology · 2003Article
- In vitro assays demonstrate that pollen tube organelles use kinesin-related motor proteins to move along microtubules.The Plant cell · 2003Article
- Association of a nonmuscle myosin II with axoplasmic organelles.Molecular biology of the cell · 2002Article
- Reconstitution of membrane transport powered by a novel dimeric kinesin motor of the Unc104/KIF1A family purified from Dictyostelium.The Journal of cell biology · 1999Article
- PAT1, a microtubule-interacting protein, recognizes the basolateral sorting signal of amyloid precursor protein.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Kinesin is a force-generating ATPase that drives the sliding movement of microtubules on glass coverslips and the movement of plastic beads along microtubules. Although kinesin is suspected to participate in microtubule-based organelle transport, the exact role it plays in this process is unclear. To address this question, we have developed a quantitative assay that allows us to determine the ability of soluble factors to promote organelle movement. Salt-washed organelles from squid axoplasm exhibited a nearly undetectable level of movement on purified microtubules. Their frequency of movement could be increased greater than 20-fold by the addition of a high speed axoplasmic supernatant. Immunoadsorption of kinesin from this supernatant decreased the frequency of organelle movement by more than 70%; organelle movements in both directions were markedly reduced. Surprisingly, antibody purified kinesin did not promote organelle movement either by itself or when it was added back to the kinesin-depleted supernatant. This result suggested that other soluble factors necessary for organelle movement were removed along with kinesin during immunoadsorption of the supernatant. A high level of organelle motor activity was recovered in a high salt eluate of the immunoadsorbent that contained only little kinesin. On the basis of these results we propose that organelle movement on microtubules involves other soluble axoplasmic factors in addition to kinesin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.