ArticleBiotechnology for biofuels2019
De novo transcriptome sequencing and analysis revealed the molecular basis of rapid fat accumulation by black soldier fly (
Article in Biotechnology for biofuels, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed.
- Multi-omics reveals an ecdysone-activated Eip75B-FABP signaling axis coordinating nutrient metabolism for development in Hermetia illucens.Journal of advanced research · 2026Article
- Comparing the Behavioral Impacts of Heavy Metals and Rare Earth Elements on Black Soldier Fly (Toxics · 2026Article
- A strategy for high-yield lauric acid production in Hermetia illucens fed with pre-fermented sweet potato-based substrate.Bioresources and bioprocessing · 2026Article
- Review
- Impact of agro-industrial by-products on nutritional value and microbiota composition of black soldier fly larvae.Frontiers in microbiology · 2026Article
- Article
- Article
- Complete mitochondrial genome of a novel black soldier fly isolate from Jordans.Scientific reports · 2025Article
- Omics in mini-livestock: a genomic perspective on the future of sustainable food systems.Frontiers in genetics · 2025Review
- Swill and Pig Manure Substrates Differentially Affected Transcriptome and Metabolome of the Black Soldier Fly Larvae.International journal of molecular sciences · 2024Article
- Diverting organic waste from landfills via insect biomanufacturing using engineered black soldier flies (Hermetia illucens).Communications biology · 2024Review
- Exploring functional metabolites and proteomics biomarkers in late-preterm and natural-born pigs.Frontiers in veterinary science · 2024Article
- Genetic structure of black soldier flies in northern Iran.PloS one · 2024Article
- Bioinformatic Surveillance Leads to Discovery of Two Novel Putative Bunyaviruses Associated with Black Soldier Fly.Viruses · 2023Article
- Chronological and Carbohydrate-Dependent Transformation of Fatty Acids in the Larvae of Black Soldier Fly Following Food Waste Treatment.Molecules (Basel, Switzerland) · 2023Article
- Black soldier fly,Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA · 2023Review
- The Variety of Applications ofBiology · 2022Review
- Modulating the Fatty Acid Profiles ofInsects · 2022Article
- First Evidence of Past and Present Interactions between Viruses and the Black Soldier Fly,Viruses · 2022Article
- Growth efficiency, intestinal biology, and nutrient utilization and requirements of black soldier fly (Hermetia illucens) larvae compared to monogastric livestock species: a review.Journal of animal science and biotechnology · 2022Review
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBlack soldier fly (BSF,
resultsThis study analyzed the dynamic state of fat content and fatty-acid composition of BSF larvae in eight different stages. The late prepupa stage exhibited the highest crude fat, with lauric acid being the main component. Therefore, to provide insight into this unexplained phenomenon, the molecular regulation of rapid fat accumulation by BSF larvae was investigated. The twelve developmental stages of BSF were selected for transcriptome analysis, including the eight stages used for investigation of fat content and fatty-acid composition. By Illumina sequencing, 218,295,450,000 nt were generated. Through assembly by Trinity, 70,475 unigenes were obtained with an average length of 1064 nt and an N50 of 1749 nt. The differentially expressed unigenes were identified by DESeq, with 9159 of them being up-regulated and 10,101 of them were down-regulated. The several putative genes that are involved in the formation of pyruvate, acetyl-CoA biosynthesis, acetyl-CoA transcription, fatty-acid biosynthesis, and triacylglycerol biosynthesis were identified. The four vital metabolic genes that are associated with fat accumulation were validated by quantitative real-time PCR (qRT-PCR). The molecular mechanism of fat accumulation in BSF was clarified in this investigation through the construction of a detailed fat accumulation model from our results.
conclusionThe study provides an unprecedented level of insight from transcriptome sequencing to reveal the crude fat accumulation mechanism in developing BSF. The finding holds considerable promise for insectival biodiesel production, and the fat content and fatty-acid composition can be altered by genetic engineering approaches in the future for the insect production industry.
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