ArticleNature communications2019
An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 55 citations in OpenAlex.
- Heterologous expression of NRPS and PKS pathways in Escherichia coli: from bottlenecks to biosynthetic platforms.Essays in biochemistry · 2026Review
- The human microbiome as a source of novel bioactive natural products: structures, bioactivities, and biosynthetic insights.Journal of natural medicines · 2026Review
- Epigenomics identifies three sources of DNA methylation inbioRxiv : the preprint server for biology · 2026Article
- Evolutionary repurposing of a metabolic thiolase complex enables antibiotic biosynthesis.Nature communications · 2026Article
- Normoxic stress enhance the secretion of adhesion-promoting membrane vesicles byJournal of oral microbiology · 2026Article
- Synergistic action of specialized metabolites from divergent biosynthesis in the human oral microbiome.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Anti-cariogenic activity of mutanocyclin, a secondary metabolite ofMicrobiology spectrum · 2025Article
- Unveiling diversity and adaptations of the wild tomato Microbiome in their center of origin in the Ecuadorian Andes.Scientific reports · 2025Article
- Synthetic Biology in Natural Product Biosynthesis.Chemical reviews · 2025Review
- Oral streptococci: modulators of health and disease.Frontiers in cellular and infection microbiology · 2024Review
- Human-associated bacteria adopt an unusual route for synthesizing 3-acetylated tetramates for environmental adaptation.Microbiome · 2023Article
- [Latest Findings on Polyketides/Non-ribosomal Peptides That Are Secondary Metabolites ofSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2023Review
- Next-generation synthetic biology approaches for the accelerated discovery of microbial natural products.Engineering microbiology · 2023Review
- Bacterial natural product discovery by heterologous expression.Journal of industrial microbiology & biotechnology · 2023Review
- Article
- Artificial intelligence accelerates the mining of bioactive small molecules from human microbiome.Clinical and translational medicine · 2022Article
- Article
- BiG-MAP: an Automated Pipeline To Profile Metabolic Gene Cluster Abundance and Expression in Microbiomes.mSystems · 2021Article
- Small molecule natural products in human nasal/oral microbiota.Journal of industrial microbiology & biotechnology · 2021Review
- Mutanofactin promotes adhesion and biofilm formation of cariogenic Streptococcus mutans.Nature chemical biology · 2021Article
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Authors and funding
16 authors at 6 institutions in 3 countries.
Funding
Abstract
Anaerobic bacteria represent an overlooked rich source of biological and chemical diversity. Due to the challenge of cultivation and genetic intractability, assessing the capability of their biosynthetic gene clusters (BGCs) for secondary metabolite production requires an efficient heterologous expression system. However, this kind of host system is still unavailable. Here, we use the facultative anaerobe Streptococcus mutans UA159 as a heterologous host for the expression of BGCs from anaerobic bacteria. A natural competence based large DNA fragment cloning (NabLC) technique was developed, which can move DNA fragments up to 40-kb directly and integrate a 73.7-kb BGC to the genome of S. mutans UA159 via three rounds of NabLC cloning. Using this system, we identify an anti-infiltration compound, mutanocyclin, from undefined BGCs from human oral bacteria. We anticipate this host system will be useful for heterologous expression of BGCs from anaerobic bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.