Evidence map›Paper›PMID 31413114›Full record

ArticleThe Journal of biological chemistry2019

A specific small-molecule inhibitor of protein kinase CδI activity improves metabolic dysfunction in human adipocytes from obese individuals.

Robert Sparks, Ashley Lui, Deena Bader, Rekha Patel, Michel Murr, Wayne Guida, Rutilio Fratti, Niketa A Patel

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. The metabolomics of a protein kinase C delta (PKCδ) knock-out mouse model.Metabolomics : Official journal of the Metabolomic Society · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Robert SparksDepartment of Biochemistry, University of Illinois, Urbana-Champaign, Illinois 61801.ORCID 0000-0002-0485-8317
Ashley LuiDepartment of Molecular Medicine, University of South Florida, Tampa, Florida 33612.ORCID 0000-0003-4090-4680
Deena BaderJames A. Haley Veterans Hospital, Tampa, Florida 33612.
Rekha PatelDepartment of Chemistry, University of South Florida, Tampa, Florida 33612.
Michel MurrSurgery Department, University of Central Florida, Orlando, Florida 32816.
Wayne GuidaDepartment of Chemistry, University of South Florida, Tampa, Florida 33612.
Rutilio FrattiDepartment of Biochemistry, University of Illinois, Urbana-Champaign, Illinois 61801.
Niketa A PatelDepartment of Molecular Medicine, University of South Florida, Tampa, Florida 33612 npatel@health.usf.edu Niketa.Patel@va.gov.
University of South Florida · USUniversity of Illinois Urbana-Champaign · USJames A. Haley Veterans' Hospital · USUniversity of Central Florida · US

Funding

Adipose stem cells' niche in obesityI01BX003836 · VA · JAMES A. HALEY VA MEDICAL CENTER · PI Niketa A. Patel · 2017 to 2026
–
BLRD VA I01 BX003836
6 · The paper itself

Abstract

The metabolic consequences and sequelae of obesity promote life-threatening morbidities. PKCδI is an important elicitor of inflammation and apoptosis in adipocytes. Here we report increased PKCδI activation via release of its catalytic domain concurrent with increased expression of proinflammatory cytokines in adipocytes from obese individuals. Using a screening strategy of dual recognition of PKCδI isozymes and a caspase-3 binding site on the PKCδI hinge domain with Schrödinger software and molecular dynamics simulations, we identified NP627, an organic small-molecule inhibitor of PKCδI. Characterization of NP627 by surface plasmon resonance (SPR) revealed that PKCδI and NP627 interact with each other with high affinity and specificity, SPR kinetics revealed that NP627 disrupts caspase-3 binding to PKCδI, and

Indexed as

AdipocytesAdipose TissueApoptosisCaspase 3Cell RespirationHumansObesityProtein Kinase C-deltaProtein Kinase InhibitorsSmall Molecule LibrariesThyrotropin-Releasing HormoneCaspase 3Protein Kinase C-deltaProtein Kinase Inhibitorspyroglutamyl-(2-propyl)histidyl-prolinamideSmall Molecule LibrariesThyrotropin-Releasing Hormoneadipocyteadipose tissueadipose tissue metabolismapoptosisinflammationmetabolic diseasemetabolic disorderNP627obesityPKC

Identifiers

PMID31413114
PMCPMC6791326
OpenAlexW2968837892

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.