Evidence map›Paper›PMID 31412956›Full record

ArticleBMC research notes2019

Kinetic assay of starvation sensitivity in yeast autophagy mutants allows for the identification of intermediary phenotypes.

Candyce M Sturgeon, Meaghan R Robinson, Molly C Penton, Deanna C Clemmer, Maria A Trujillo, Ambar U Khawaja, Verónica A Segarra

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In one paragraph

Article in BMC research notes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Candyce M SturgeonDepartment of Biology, High Point University, One University Parkway, High Point, NC, 27268, USA.
Meaghan R RobinsonDepartment of Biology, High Point University, One University Parkway, High Point, NC, 27268, USA.
Molly C PentonDepartment of Biology, High Point University, One University Parkway, High Point, NC, 27268, USA.
Deanna C ClemmerDepartment of Biology, High Point University, One University Parkway, High Point, NC, 27268, USA.
Maria A TrujilloDepartment of Biology, High Point University, One University Parkway, High Point, NC, 27268, USA.
Ambar U KhawajaInternational Baccalaureate Program, High Point Central High School, High Point, NC, 27262, USA.
Verónica A SegarraDepartment of Biology, High Point University, One University Parkway, High Point, NC, 27268, USA. vsegarra@highpoint.edu.ORCID http://orcid.org/0000-0001-6914-8271

Funding

High Point University Research Advancement Grant 17-065
6 · The paper itself

Abstract

objectiveA classical method to quantitatively determine the starvation sensitivity phenotype of autophagy mutant budding yeast strains is to starve them for a period of time and then to assess the proportion of cells that retain the ability to form colonies when the availability of nutrients is restored. The readout of this colony-formation assay is generally evaluated after a fixed period of time following the restoration of nutrients, so that it can be considered an endpoint assay. One drawback we have identified is the inability to characterize subtle intermediary phenotypes that are detectable at the molecular level but fail to reach statistical significance in the colony formation experiment. We set out to determine whether a more dynamic measurement of growth during recovery after starvation would increase the sensitivity with which we are able to detect partial loss-of-function phenotypes.

resultsWe describe a 96-well plate-based assay to kinetically assess starvation sensitivity in budding yeast that allows for the quantitative detection of very modest starvation sensitivity phenotypes with statistical significance in autophagy mutant yeast strains lacking the ATG27 gene.

Indexed as

MutationStarvationAutophagyAutophagy-Related ProteinsKineticsPhenotypeSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsATG27 protein, S cerevisiaeAutophagy-Related ProteinsSaccharomyces cerevisiae Proteins96-well plate assayAtg27AutophagyBudding yeastHigh-throughput starvation sensitivity assayStarvation sensitivity

Identifiers

PMID31412956
PMCPMC6694668

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.