ArticleStem cell research & therapy2019
Design of experiments to assess the effect of culture parameters on the osteogenic differentiation of human adipose stromal cells.
Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
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- Statistical optimization of hydrazone-crosslinked hyaluronic acid hydrogels for protein delivery.Journal of materials chemistry. B · 2024Article
- The Impact of Culture Variables on a 3D Human In Vitro Bone Remodeling Model: A Design of Experiments Approach.Advanced healthcare materials · 2023Article
- Micronutrient optimization for tissue engineered articular cartilage production of type II collagen.Frontiers in bioengineering and biotechnology · 2023Article
- Development of a fibrin-mediated gene delivery system for the treatment of cystinosis via design of experiment.Scientific reports · 2022Article
- Review
- Bone regeneration in rat calvarial defects using dissociated or spheroid mesenchymal stromal cells in scaffold-hydrogel constructs.Stem cell research & therapy · 2021Article
- Critical Analysis of cGMP Large-Scale Expansion Process in Bioreactors of Human Induced Pluripotent Stem Cells in the Framework of Quality by Design.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- Design of experiments for the optimization of SOFI super-resolution microscopy imaging.Biomedical optics express · 2021Article
- Continuous Electrical Stimulation Affects Initial Growth and Proliferation of Adipose-Derived Stem Cells.Biomedicines · 2020Article
- Influence of platelet storage time on human platelet lysates and platelet lysate-expanded mesenchymal stromal cells for bone tissue engineering.Stem cell research & therapy · 2020Article
- Recent advances in culture medium design for enhanced production of monoclonal antibodies in CHO cells: A comparative study of machine learning and systems biology approaches.Biotechnology advancesReview
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Authors and funding
6 authors.
Funding
Abstract
backgroundHuman adipose-derived stromal cells (hASCs) have been gaining increasing popularity in regenerative medicine thanks to their multipotency, ease of collection, and efficient culture. Similarly to other stromal cells, their function is particularly sensitive to the culture conditions, including the composition of the culture medium. Given the large number of parameters that can play a role in their specification, the rapid assessment would be beneficial to allow the optimization of their culture parameters.
methodHerein we used the design of experiments (DOE) method to rapidly screen the influence and relevance of several culture parameters on the osteogenic differentiation of hASCs. Specifically, seven cell culture parameters were selected for this study based on a literature review. These parameters included the source of hASCs (the different providers having different methods for processing the cells prior to their external use), the source of serum (fetal bovine serum vs. human platelet lysate), and several soluble osteoinductive factors, including dexamethasone and a potent growth factor, the bone morphogenetic protein-9 (BMP-9). The expression of alkaline phosphatase was quantified as a readout for the osteogenic differentiation of hASCs.
resultsThe DOE analysis enabled to classify the seven studied parameters according to their relative influence on the osteogenic differentiation of hASCs. Notably, the source of serum was found to have a major effect on the osteogenic differentiation of hASCs as well as their origin (different providers) and the presence of L-ascorbate-2-phosphate and BMP-9.
conclusionThe DOE-based screening is a valuable approach for the classification of the impact of several cell culture parameters on the osteogenic differentiation of hASCs.
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