ArticlePloS one2019
Antibiotic resistant bacteria and commensal fungi are common and conserved in the mosquito microbiome.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
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- Effects of Wolbachia removal on microbial composition and diversity in Aedes albopictus: implication of using wAlbB for discriminating irradiation-based sterile and wild males.Infectious diseases of poverty · 2025Article
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- Fungal communities in Florida salt marsh mosquito midguts vary between species and over time but have low structure.Frontiers in immunology · 2025Article
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- The dynamics of the midgut microbiome inPNAS nexus · 2024Article
- Rapid Phenotypic Antibiotics Susceptibility Analysis by a 3D Printed Prototype.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Interkingdom interactions shape the fungal microbiome of mosquitoes.Animal microbiome · 2024Article
- The Most Common Multi-Drug Resistant Bacteria Associated with Hospital Infections, in Urmia, Iran.Iranian journal of pathology · 2024Article
- Introducing an environmental microbiome to axenicApplied and environmental microbiology · 2023Article
- The mosquito Aedes aegypti requires a gut microbiota for normal fecundity, longevity and vector competence.Communications biology · 2023Article
- Three species of axenic mosquito larvae recruit a shared core of bacteria in a common garden experiment.Applied and environmental microbiology · 2023Article
- Role of the Microbiome inViruses · 2023Review
- Bacterial communities of Aedes aegypti mosquitoes differ between crop and midgut tissues.PLoS neglected tropical diseases · 2023Article
- Of Cockroaches and Symbionts: Recent Advances in the Characterization of the Relationship betweenLife (Basel, Switzerland) · 2022Review
- Analyzing gut microbiota composition in individualiScience · 2021Article
- Interkingdom Gut Microbiome and Resistome of the CockroachmSystems · 2021Article
- Considerations for mosquito microbiome research from the Mosquito Microbiome Consortium.Microbiome · 2021Review
- Insects, Rodents, and Pets as Reservoirs, Vectors, and Sentinels of Antimicrobial Resistance.Antibiotics (Basel, Switzerland) · 2021Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emerging and increasing prevalence of bacterial antibiotic resistance is a significant public health challenge. To begin to tackle this problem, it will be critical to not only understand the origins of this resistance but also document environmental reservoirs of antibiotic resistance. In this study we investigated the possibility that both colony and field caught mosquitoes could harbor antibiotic resistant bacteria. Specifically, we characterized the antibiotic resistant bacterial populations from colony-reared Aedes aegypti larvae and adults and two field caught mosquito species Coquillettidia perturbans and Ochlerotatus canadensis. The cultured bacterial populations were dominated by isolates belonging to the class Gammaproteobacteria. Among the antibiotic resistant populations, we found bacteria resistant to carbenicillin, kanamycin, and tetracycline, including bacteria resistant to a cocktail of all three antibiotics in combination. The antibiotic resistant bacteria were numerically rare, at most 5% of total cell counts. Isolates were characterized by 16S rRNA gene sequencing, and clustering into Operational Taxonomic Units (OTUs; 99% sequence identity). 27 antibiotic resistant OTUs were identified, although members of an OTU did not always share the same resistance profile. This suggests the clustering was either not sensitive enough to distinguish different bacteria taxa or different antibiotic resistant sub-populations exist within an OTU. Finally, the antibiotic selection opened up a niche to culture mosquito-associated fungi, and 10 fungal OTUs (28S rRNA gene sequencing) were identified. Two fungal OTUs both classified to the class Microbotryomycetes were commonly identified in the field-caught mosquitoes. Thus, in this study we demonstrate that antibiotic resistant bacteria and certain fungi are common and conserved mosquito microbiome members. These observations highlight the potential of invertebrates to serve as vehicles for the spread of antibiotic resistance throughout the environment.
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