ArticleJournal of applied oral science : revista FOB2019
Role of extracellular DNA in Enterococcus faecalis biofilm formation and its susceptibility to sodium hypochlorite.
Article in Journal of applied oral science : revista FOB, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Translating microbial knowledge into clinical biofilm therapies.Open medicine (Warsaw, Poland) · 2026Review
- Using stochastic cellular automata to model and define sufficient conditions for the survival of Enterococcus faecalis biofilms with the pCF10 plasmid under erythromycin treatment.PLoS computational biology · 2025Article
- The effect of the enzymes trypsin and DNase I on the antimicrobial efficiency of root canal irrigation solutions.Odontology · 2024Article
- Strategies and mechanisms targetingFrontiers in cellular and infection microbiology · 2024Review
- An Overview of the Factors Involved in Biofilm Production by theInternational journal of molecular sciences · 2023Review
- Revisiting ESKAPE Pathogens: virulence, resistance, and combating strategies focusing on quorum sensing.Frontiers in cellular and infection microbiology · 2023Review
- Strengthening of enterococcal biofilms by Esp.PLoS pathogens · 2022Article
- Antibacterial and anti-biofilm activity of diarylureas againstFrontiers in microbiology · 2022Article
- Antimicrobial effects of different concentrations of simvastatin versus triple antibiotic paste onJournal of dental research, dental clinics, dental prospects · 2022Article
- Silver nanoparticles in endodontics: recent developments and applications.Restorative dentistry & endodontics · 2021Review
- High concentrations of hypochlorous acid-based disinfectant in the environment reduced the load of SARS-CoV-2 in nucleic acid amplification testing.Electrophoresis · 2021Article
- Characterization, Antimicrobial Effects, and Cytocompatibility of a Root Canal Sealer Produced by Pozzolan Reaction between Calcium Hydroxide and Silica.Materials (Basel, Switzerland) · 2021Article
- Single DNase or Proteinase Treatment Induces Change in Composition and Structural Integrity of Multispecies Oral Biofilms.Antibiotics (Basel, Switzerland) · 2021Article
- Understanding the Matrix: The Role of Extracellular DNA in Oral Biofilms.Frontiers in oral health · 2021Review
- Effects of ClpP protease on biofilm formation of Enterococcus faecalis.Journal of applied oral science : revista FOB · 2021Article
- The Ability of a Concentrated Surfactant Gel to Reduce an Aerobic, Anaerobic and Multispecies Bacterial Biofilm In Vitro.Advances in experimental medicine and biology · 2021Article
- Characterization of Enterococcus faecalis in different culture conditions.Scientific reports · 2020Article
- Combined Effect of Melittin and DNase onMaterials (Basel, Switzerland) · 2020Article
- Combined DNase and Proteinase Treatment Interferes with Composition and Structural Integrity of Multispecies Oral Biofilms.Journal of clinical medicine · 2020Article
- Effect of a calcium hydroxide-based intracanal medicament containing N-2-methyl pyrrolidone as a vehicle against Enterococcus faecalis biofilm.Journal of applied oral science : revista FOB · 2020Article
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThis study investigated the role of extracellular deoxyribonucleic acid (eDNA) on Enterococcus faecalis ( E. faecalis ) biofilm and the susceptibility of E. faecalis to sodium hypochlorite (NaOCl). METHODOLOGY: E. faecalis biofilm was formed in bovine tooth specimens and the biofilm was cultured with or without deoxyribonuclease (DNase), an inhibitor of eDNA. Then, the role of eDNA in E. faecalis growth and biofilm formation was investigated using colony forming unit (CFUs) counting, eDNA level assay, crystal violet staining, confocal laser scanning microscopy, and scanning electron microscopy. The susceptibility of E. faecalis biofilm to low (0.5%) or high (5%) NaOCl concentrations was also analyzed by CFU counting.
resultsCFUs and biofilm formation decreased significantly with DNase treatment (p<0.05). The microstructure of DNase-treated biofilms exhibited less structured features when compared to the control. The volume of exopolysaccharides in the DNase-treated biofilm was significantly lower than that of control (p<0.05). Moreover, the CFUs, eDNA level, biofilm formation, and exopolysaccharides volume were lower when the biofilm was treated with DNase de novo when compared to when DNase was applied to matured biofilm (p<0.05). E. faecalis in the biofilm was more susceptible to NaOCl when it was cultured with DNase (p<0.05). Furthermore, 0.5% NaOCl combined with DNase treatment was as efficient as 5% NaOCl alone regarding susceptibility (p>0.05).
conclusionsInhibition of eDNA leads to decrease of E. faecalis biofilm formation and increase of susceptibility of E. faecalis to NaOCl even at low concentrations. Therefore, our results suggest that inhibition of eDNA would be beneficial in facilitating the efficacy of NaOCl and reducing its concentration.
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