ArticleScientific reports2019
Development and evaluation of double gene transgenic cotton lines expressing Cry toxins for protection against chewing insect pests.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A combination of cry (Cry2Ab) and plant lectin (PTA) genes confers resistance against major sucking and chewing insect pests of cotton.Molecular biology reports · 2025Article
- Molecular Characterization and Efficacy Evaluation of Transgenic Maize HarboringPlants (Basel, Switzerland) · 2023Article
- Silencing ofInsects · 2022Article
- Development and evaluation of triple gene transgenic cotton lines expressing three genes (Cry1Ac-Cry2Ab-EPSPS) for lepidopteran insect pests and herbicide tolerance.Scientific reports · 2022Article
- Double-Gene Copromoting Expression Analysis in tPA/GH Transgenic Goat Mammary Epithelial Cells and Thrombolytic Activity of tPA In Vitro.BioMed research international · 2022Article
- Biotechnological Approaches for Host Plant Resistance to Insect Pests.Frontiers in genetics · 2022Review
- Article
- Development of broad-spectrum and sustainable resistance in cotton against major insects through the combination of Bt and plant lectin genes.Plant cell reports · 2021Article
- Development of event-specific detection method for identification of insect resistant NIBGE-1601 cotton harboring double gene Cry1Ac-Cry2Ab construct.Scientific reports · 2021Article
- Efficient Transformation ofFrontiers in plant science · 2021Article
- Exploitation of NovelFrontiers in microbiology · 2021Article
- Effect of Urea Spray on Boll Shell Insecticidal Protein Content in Bt Cotton.Frontiers in plant science · 2021Article
- Genetic engineering of crops for insect resistance: An overview.Journal of biosciences · 2020Article
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Authors and funding
15 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Cotton is the main fiber producing crop globally, with a significant impact on the economy of Pakistan. Bt cotton expressing a Cry1Ac gene is grown over a large area in Pakistan, however, there is a major concern that bollworms may develop resistance. Here we have used a durable resistance strategy against bollworms by developing a double gene construct containing Cry1Ac and Cry2Ab (pGA482-12R) for cotton transformation. Both Cry toxin genes have been cloned in the same T-DNA borders and transferred successfully into cotton via Agrobacterium-mediated transformation. Both genes are expressed in transgenic cotton plants and is likely to help breeders in developing new cotton cultivars by incorporating these genes in cotton lines having no Bt genes or expressing Cry1Ac gene (Mon 531). Positive transgenic cotton was identified by PCR using specific primers for the amplification of both Cry1Ac and Cry2Ab genes. Cry1Ac and Cry2Ab expression was confirmed with an immunostrip test and quantified using ELISA that showed significant spatio-temporal expression of Cry2Ab ranging from 3.28 to 7.72 µg/g of the tissue leaf. Insect bioassay with army worm (Spodoptera litura) was performed to check the efficacy of NIBGE (National Institute for Biotechnology and Genetic Engineering) double gene transgenic cotton plants and up to 93% insect mortality was observed.
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