ArticleScientific reports2019
Transcriptomic Profiling of Adipose Derived Stem Cells Undergoing Osteogenesis by RNA-Seq.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Molecular plasticity of LAMA3 across the disease spectrum: pathogenic mechanisms and clinical translation.Histochemistry and cell biology · 2026Pooled it
- The investigation of molecular mechanisms influencing variations of sternum calcification in ducks by integrating transcriptomic and metabolomic analyses.Poultry science · 2026Article
- 14-3-3γ Knockdown promotes matrix mineralization in human mesenchymal stromal cells.Cell death & disease · 2026Article
- Brushite-modified titanium surface elicits early osteointegrative gene expression signatures in human mesenchymal stem cells: insights from NGS analysis.Frontiers in bioengineering and biotechnology · 2026Article
- An updated narrative review on revolutionizing erectile dysfunction treatment: the crucial role of trophic factors in Adipose-Derived stem cell therapy.BMC urology · 2025Review
- Dataset of transcriptomic changes induced by naringenin treatment in MCF-7 breast cancer cells.Data in brief · 2025Article
- The role of lncRNA in the differentiation of adipose-derived stem cells: from functions to mechanism.Journal of molecular medicine (Berlin, Germany) · 2025Review
- Comparative Analysis of mRNA and lncRNA Expression Profiles in Testicular Tissue of Sexually Immature and Sexually Mature Mongolian Horses.Animals : an open access journal from MDPI · 2024Article
- Transcriptomic Analysis of Mineralized Adipose-Derived Stem Cell Tissues for Calcific Valve Disease Modelling.International journal of molecular sciences · 2024Article
- Differential regulation of apoptosis-related genes during long-term culture and differentiation of canine adipose-derived stem cells - a functional bioinformatical analysis.Frontiers in genetics · 2024Article
- Investigating the association between serum ADAM/ADAMTS levels and bone mineral density by mendelian randomization study.BMC genomics · 2023Article
- Transcriptome changes during osteogenesis of porcine mesenchymal stem cells derived from different types of synovial membranes and genetic background.Scientific reports · 2023Article
- Betaine regulates adipogenic and osteogenic differentiation of hAD-MSCs.Molecular biology reports · 2023Article
- Decreased CRISPLD2 expression impairs osteogenic differentiation of human mesenchymal stem cells during in vitro expansion.Journal of cellular physiology · 2023Article
- Combining single-cell transcriptomics and CellTagging to identify differentiation trajectories of human adipose-derived mesenchymal stem cells.Stem cell research & therapy · 2023Article
- Modulation of osteogenic differentiation by Escherichia coli-derived recombinant bone morphogenetic protein-2.AMB Express · 2022Article
- Understanding the Transcriptomic Landscape to Drive New Innovations in Musculoskeletal Regenerative Medicine.Current osteoporosis reports · 2022Review
- Single-Cell Transcriptome Profiling Signatures and Alterations of Microglia Associated With Glioblastoma Associate Microglia Contribution to Tumor Formation.Pathology oncology research : POR · 2022Article
- ADAMTS6: Emerging roles in cardiovascular, musculoskeletal and cancer biology.Frontiers in molecular biosciences · 2022Review
- Mouse Embryonic Fibroblast Adipogenic Potential: A Comprehensive Transcriptome Analysis.Adipocyte · 2021Article
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Authors and funding
5 authors at 3 institutions in 1 country.
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Abstract
Adipose-derived stromal/stem cells (ASCs) are multipotent in nature that can be differentiated into various cells lineages such as adipogenic, osteogenic, and chondrogenic. The commitment of a cell to differentiate into a particular lineage is regulated by the interplay between various intracellular pathways and their resultant secretome. Similarly, the interactions of cells with the extracellular matrix (ECM) and the ECM bound growth factors instigate several signal transducing events that ultimately determine ASC differentiation. In this study, RNA-sequencing (RNA-Seq) was performed to identify the transcriptome profile of osteogenic induced ASCs to understand the associated genotype changes. Gene ontology (GO) functional annotations analysis using Database for Annotation Visualization and Integrated Discovery (DAVID) bioinformatics resources on the differentially expressed genes demonstrated the enrichment of pathways mainly associated with ECM organization and angiogenesis. We, therefore, studied the expression of genes coding for matrisome proteins (glycoproteins, collagens, proteoglycans, ECM-affiliated, regulators, and secreted factors) and ECM remodeling enzymes (MMPs, integrins, ADAMTSs) and the expression of angiogenic markers during the osteogenesis of ASCs. The upregulation of several pro-angiogenic ELR+ chemokines and other angiogenic inducers during osteogenesis indicates the potential role of the secretome from differentiating ASCs in the vascular development and its integration with the bone tissue. Furthermore, the increased expression of regulatory genes such as CTNNB1, TGBR2, JUN, FOS, GLI3, and MAPK3 involved in the WNT, TGF-β, JNK, HedgeHog and ERK1/2 pathways suggests the regulation of osteogenesis through interplay between these pathways. The RNA-Seq data was also validated by performing QPCR on selected up- and down-regulated genes (COL10A1, COL11A1, FBLN, FERMT1, FN1, FOXF1, LAMA3, LAMA4, LAMB1, IGF1, WNT10B, MMP1, MMP3, MMP16, ADAMTS6, and ADAMTS14).
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