ArticleScientific reports2019
Multi-foci laser microfabrication of 3D polymeric scaffolds for stem cell expansion in regenerative medicine.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Nanoengineered 3D culture substrate enables superior persistence and polyclonal engraftment of genetically engineered hematopoietic stem cells.Cell stem cell · 2026Article
- Engineering macrophage responses through 3D scaffold microarchitecture.Materials today. Bio · 2025Article
- A 3D millifluidic model of a dermal perivascular microenvironment on a chip.Lab on a chip · 2025Article
- The importance of 3D fibre architecture in cancer and implications for biomaterial model design.Nature reviews. Cancer · 2024Review
- Article
- Article
- Auxeticity as a Mechanobiological Tool to Create Meta-Biomaterials.ACS applied bio materials · 2023Review
- Article
- Integration of Biofunctional Molecules into 3D-Printed Polymeric Micro-/Nanostructures.Polymers · 2022Article
- Whole transcriptomic analysis of mesenchymal stem cells cultured in Nichoid micro-scaffolds.Frontiers in bioengineering and biotechnology · 2022Article
- Multiphoton Laser Fabrication of Hybrid Photo-Activable Biomaterials.Sensors (Basel, Switzerland) · 2021Review
- Article
- Neural precursors cells expanded in a 3D micro-engineered niche present enhanced therapeutic efficacyNanotheranostics · 2021Article
- Characterization of Mesenchymal Stem Cell Differentiation within Miniaturized 3D Scaffolds through Advanced Microscopy Techniques.International journal of molecular sciences · 2020Article
- Dissecting the Effect of a 3D Microscaffold on the Transcriptome of Neural Stem Cells with Computational Approaches: A Focus on Mechanotransduction.International journal of molecular sciences · 2020Article
- Article
- Femtosecond Laser-Based Integration of Nano-Membranes into Organ-on-a-Chip Systems.Materials (Basel, Switzerland) · 2020Article
- Nonlinear Optical Microscopy: From Fundamentals to Applications in Live Bioimaging.Frontiers in bioengineering and biotechnology · 2020Review
- Bioengineering tools to speed up the discovery and preclinical testing of vaccines for SARS-CoV-2 and therapeutic agents for COVID-19.Theranostics · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High quality large scale fabrication of cellular scaffolds, with three-dimensional resolution comparable to cell size, is an important task to enable regenerative medicine applications with stem cells. We are using two-photon polymerization to produce our stem cell culture substrate called Nichoid, which we already demonstrated capable of stimulating cell proliferation while maintaining their stemness, without the need of dangerous additives. Parallelization of this technique can be achieved with the use of a spatial light modulator: here we show the results obtained combining this device with fast linear stages to produce Nichoid-covered substrates by two-photon polymerization. The well-polymerized structures confirm that this approach is particularly convenient for porous structures, and allows a significant time saving by a factor of almost five, with minor design adjustments. A Live & Dead assay was performed on mesenchymal stem cells cultured into the Nichoid microstructures in order to verify that no difference in cell viability is present, compared to microstructures fabricated by a single focus. This parallel setup opens the possibility to obtain a much larger number of microstructured substrates, that are essential to test new stem cell-based therapies. This approach can be also used for the fast fabrication of other kinds of cell culture devices.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.