Evidence map›Paper›PMID 31409793›Full record

ArticleNature communications2019

A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier.

Astgik Petrosyan, Paolo Cravedi, Valentina Villani, Andrea Angeletti, Joaquin Manrique, Alessandra Renieri, Roger E De Filippo, Laura Perin, Stefano Da Sacco

Erratum issuedOpen access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 114 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
114citing papers in PubMed, 1 pooled it
10.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

114 citing papers in PubMed, 1 synthesis or guideline pooled it, 223 citations in OpenAlex.

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54 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 3 countries.

Astgik PetrosyanGOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics in Urology, Saban Research Institute, Division of Urology, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Paolo CravediDivision of Nephrology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Valentina VillaniGOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics in Urology, Saban Research Institute, Division of Urology, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Andrea AngelettiDepartment of Experimental, Diagnostic and Specialty Medicine, Nephrology, Dialysis and Renal Transplant Unit, S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy.ORCID http://orcid.org/0000-0002-6121-5326
Joaquin ManriqueNephrology Service, Complejo Hospitalario de Navarra, Pamplona, Spain.
Alessandra RenieriGenetica Medica, Azienda Ospedaliera Universitaria Senese, Siena, Italy.ORCID http://orcid.org/0000-0002-0846-9220
Roger E De FilippoGOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics in Urology, Saban Research Institute, Division of Urology, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Laura Perin *GOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics in Urology, Saban Research Institute, Division of Urology, Children's Hospital Los Angeles, Los Angeles, CA, USA. lperin@chla.usc.edu.
Stefano Da Sacco *GOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics in Urology, Saban Research Institute, Division of Urology, Children's Hospital Los Angeles, Los Angeles, CA, USA. sdasacco@chla.usc.edu.ORCID http://orcid.org/0000-0001-9654-6822
University of Southern California · USChildren's Hospital of Los Angeles · USAzienda Ospedaliera Universitaria Senese · ITComplejo Hospitalario de Navarra · ESIcahn School of Medicine at Mount Sinai · USIRCCS Azienda Ospedliero-Universitaria di Bologna Policlinico di Sant'Orsola · IT

Funding

Mechanisms of complement-mediated podocyte injury in FSGSR01DK119431 · NIDDK · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI CRAVEDI, PAOLO · 2019 to 2021
$763k
NIDDK NIH HHS R01 DK119431
6 · The paper itself

Abstract

In this work we model the glomerular filtration barrier, the structure responsible for filtering the blood and preventing the loss of proteins, using human podocytes and glomerular endothelial cells seeded into microfluidic chips. In long-term cultures, cells maintain their morphology, form capillary-like structures and express slit diaphragm proteins. This system recapitulates functions and structure of the glomerulus, including permselectivity. When exposed to sera from patients with anti-podocyte autoantibodies, the chips show albuminuria proportional to patients' proteinuria, phenomenon not observed with sera from healthy controls or individuals with primary podocyte defects. We also show its applicability for renal disease modeling and drug testing. A total of 2000 independent chips were analyzed, supporting high reproducibility and validation of the system for high-throughput screening of therapeutic compounds. The study of the patho-physiology of the glomerulus and identification of therapeutic targets are also feasible using this chip.

Indexed as

Lab-On-A-Chip DevicesAlbuminsAlbuminuriaCells, ImmobilizedEndothelial CellsHumansKidney GlomerulusMaleNephritis, HereditaryPodocytesAlbumins

Identifiers

PMID31409793
PMCPMC6692336
OpenAlexW2967299926

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.