ArticleMicrobial cell factories2019
Enhanced acid-stress tolerance in Lactococcus lactis NZ9000 by overexpression of ABC transporters.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 79 citations in OpenAlex.
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- Membrane-embedded CdaA is required for efficient synthesis of second messenger cyclic di-AMP.Communications biology · 2024Article
- Identification of acidic stress-responsive genes and acid tolerance engineering in Synechococcus elongatus PCC 7942.Applied microbiology and biotechnology · 2024Article
- Characterization of Mild Acid Stress Response in an Engineered Acid-TolerantMicroorganisms · 2024Article
- Strategies to enhance stress tolerance in lactic acid bacteria across diverse stress conditions.World journal of microbiology & biotechnology · 2024Review
- Expression of stress responsive genes enablesFrontiers in microbiology · 2024Article
- Effect of salinity on genes involved in the stress response in mangrove soils.Antonie van Leeuwenhoek · 2023Article
- Genomic Plasticity of Acid-Tolerant Phenotypic Evolution in Acetobacter pasteurianus.Applied biochemistry and biotechnology · 2023Article
- Enhancement of acid tolerance of Escherichia coli by introduction of molecule chaperone CbpA from extremophile.World journal of microbiology & biotechnology · 2023Article
- Recent advances in genetic tools for engineering probiotic lactic acid bacteria.Bioscience reports · 2023Article
- Metabolic engineering ofFrontiers in bioengineering and biotechnology · 2023Article
- Identification of a major facilitator superfamily protein that is beneficial to L-lactic acid production by Bacillus coagulans at low pH.BMC microbiology · 2022Article
- Characterization of two new strains of Lactococcus lactis for their probiotic efficacy over commercial synbiotics consortia.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Ribosome Profiling Reveals Genome-Wide Cellular Translational Regulation inFoods (Basel, Switzerland) · 2022Article
- Engineering robust microorganisms for organic acid production.Journal of industrial microbiology & biotechnology · 2022Review
- Inhibitor tolerance and bioethanol fermentability of levoglucosan-utilizingSynthetic and systems biotechnology · 2021Article
- Genome-Wide Transcriptomic Analysis ofJournal of microbiology and biotechnology · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMicrobial cell factories are widely used in the production of acidic products such as organic acids and amino acids. However, the metabolic activity of microbial cells and their production efficiency are severely inhibited with the accumulation of intracellular acidic metabolites. Therefore, it remains a key issue to enhance the acid tolerance of microbial cells. In this study, we investigated the effects of four ATP-binding cassette (ABC) transporters on acid stress tolerance in Lactococcus lactis.
resultsOverexpressing the rbsA, rbsB, msmK, and dppA genes exhibited 5.8-, 12.2-, 213.7-, and 5.2-fold higher survival rates than the control strain, respectively, after acid shock for 3 h at pH 4.0. Subsequently, transcriptional profile alterations in recombinant strains were analyzed during acid stress. The differentially expressed genes associated with cold-shock proteins (csp), fatty acid biosynthesis (fabH), and coenzyme A biosynthesis (coaD) were up-regulated in the four recombinant strains during acid stress. Additionally, some genes were differentially expressed in specific recombinant strains. For example, in L. lactis (RbsB), genes involved in the pyrimidine biosynthetic pathway (pyrCBDEK) and glycine or betaine transport process (busAA and busAB) were up-regulated during acid stress, and the argG genes showed up-regulations in L. lactis (MsmK). Finally, we found that overexpression of the ABC transporters RbsB and MsmK increased intracellular ATP concentrations to protect cells against acidic damage in the initial stage of acid stress. Furthermore, L. lactis (MsmK) consistently maintained elevated ATP concentrations under acid stress.
conclusionsThis study elucidates the common and specific mechanisms underlying improved acid tolerance by manipulating ABC transporters and provides a further understanding of the role of ABC transporters in acid-stress tolerance.
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