ArticleBMC microbiology2019
Development and evaluation of a rapid and sensitive RPA assay for specific detection of Vibrio parahaemolyticus in seafood.
Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Establishment of methods for visual and rapid detection of piscine lactococcosis based on isothermal recombinase polymerase amplification.BMC veterinary research · 2026Article
- A miniaturized, frugal RPA assay for genus-level detection of Paracoccidioides spp. in resource-limited endemic settings.PLoS neglected tropical diseases · 2026Article
- Development of a recombinase polymerase amplification nucleic acid lateral flow assay for detecting Streptococcus suis serotype 2 in pork.Scientific reports · 2025Article
- A 3D-Printed Integrated Handheld Biosensor for the Detection ofFoods (Basel, Switzerland) · 2024Article
- Development of Multienzyme Isothermal Rapid Amplification (MIRA) Combined with Lateral-Flow Dipstick (LFD) Assay to Detect Species-SpecificPathogens (Basel, Switzerland) · 2024Article
- Effect of food matrix on rapid detection of Vibrio parahaemolyticus in aquatic products based on toxR gene.World journal of microbiology & biotechnology · 2023Article
- Article
- Moving towards improved surveillance and earlier diagnosis of aquatic pathogens: From traditional methods to emerging technologies.Reviews in aquaculture · 2022Review
- CE-RAA-CRISPR Assay: A Rapid and Sensitive Method for DetectingFoods (Basel, Switzerland) · 2022Article
- Capture and identification of bacteria from fish muscle based on immunomagnetic beads and MALDI-TOF MS.Food chemistry: X · 2022Article
- Rapid Detection ofJournal of microbiology and biotechnology · 2022Article
- Rapid Detection ofFrontiers in cellular and infection microbiology · 2021Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVibrio parahaemolyticus (V. parahaemolyticus) is a leading cause of food poisoning and is of great importance to public health due to the frequency and seriousness of the diseases. The simple, timely and efficient detection of this pathogen is a major concern worldwide. In this study, we established a simple and rapid method based on recombinase polymerase amplification (RPA) for the determination of V. parahaemolyticus. According to the gyrB gene sequences of V. parahaemolyticus available in GenBank, specific primers and an exo probe were designed for establishing real-time recombinase polymerase amplification (real-time RPA).
resultsThe real-time RPA reaction was performed successfully at 38 °C, and results were obtained within 20 min. The method only detected V. parahaemolyticus and did not show cross-reaction with other bacteria, exhibiting a high level of specificity. The study showed that the detection limit (LOD) of real-time RPA was 1.02 × 10
conclusionThis study describes a simple, rapid, and reliable method for the detection of V. parahaemolyticus, which could potentially be applied in the research laboratory and disease diagnosis.
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