ArticlePLoS pathogens2019
Cyclic-di-GMP binds to histidine kinase RavS to control RavS-RavR phosphotransfer and regulates the bacterial lifestyle transition between virulence and swimming.
Article in PLoS pathogens, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- DrdR Negatively Modulates the Expression of Flagellar Genes via Interaction With FleQ in Xanthomonas campestris.Molecular plant pathology · 2026Article
- Spatiotemporal coordination of virulence, metabolism, and stress responses shapes infection dynamics ofApplied and environmental microbiology · 2026Article
- A chemical bactericide dioctyldiethylenetriamine (Xinjunan) exerts a non-lethal effect by inhibiting RpfG activity to regulate the quorum sensing system.PLoS pathogens · 2026Article
- Isolation, Identification and Pathogenicity Analysis of Proteus mirabilis in Cynomolgus Monkey From Yunnan, China.Veterinary medicine and science · 2026Article
- The Transcriptional Regulator Crt1 is Involved in The Pathogenic Lifestyle ofPolish journal of microbiology · 2026Article
- Osmotic and pH Stress-Responsive Two-Component System, OmpR/EnvZ, Modulates Type III Secretion, Biofilm Formation, Swimming Motility and Virulence in Acidovorax citrulli xjL12.Molecular plant pathology · 2025Article
- Autoinducer-2 enhances the defense ofmBio · 2025Article
- Crosstalk between cyclic-di-guanosine monophosphate and the sensor kinase MtrB regulates MtrA-dependent genes, bacterial growth, biofilm formation and lysosomal trafficking ofMicrobiology (Reading, England) · 2025Article
- A novel two-component system contributing the catabolism of c-di-GMP influences virulence inFrontiers in microbiology · 2025Article
- Cyclic Diguanylate in the Wild: Roles During Plant and Animal Colonization.Annual review of microbiology · 2024Review
- Stenotrophomonas maltophilia uses a c-di-GMP module to sense the mammalian body temperature during infection.PLoS pathogens · 2024Article
- A cyclic di-GMP-binding adaptor protein interacts with a N5-glutamine methyltransferase to regulate the pathogenesis in Xanthomonas citri subsp. citri.Molecular plant pathology · 2024Article
- Review
- Dual Regulatory Role Exerted by Cyclic Dimeric GMP To Control FsnR-Mediated Bacterial Swimming.mBio · 2022Article
- BDSF Is a Degradation-Prone Quorum-Sensing Signal Detected by the Histidine Kinase RpfC ofApplied and environmental microbiology · 2022Article
- Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.Biomolecules · 2021Review
- The predatory soil bacterium Lysobacter reprograms quorum sensing system to regulate antifungal antibiotic production in a cyclic-di-GMP-independent manner.Communications biology · 2021Article
- Network-based analysis of virulence factors for uncovering Aeromonas veronii pathogenesis.BMC microbiology · 2021Article
- PAS domains in bacterial signal transduction.Current opinion in microbiology · 2021Review
- An improved, versatile and efficient modular plasmid assembly system for expression analyses of genes in Xanthomonas oryzae.Molecular plant pathology · 2021Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The two-component signalling system (TCS) comprising a histidine kinase (HK) and a response regulator (RR) is the predominant bacterial sense-and-response machinery. Because bacterial cells usually encode a number of TCSs to adapt to various ecological niches, the specificity of a TCS is in the centre of regulation. Specificity of TCS is defined by the capability and velocity of phosphoryl transfer between a cognate HK and a RR. Here, we provide genetic, enzymology and structural data demonstrating that the second messenger cyclic-di-GMP physically and specifically binds to RavS, a HK of the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. The [c-di-GMP]-RavS interaction substantially promotes specificity between RavS and RavR, a GGDEF-EAL domain-containing RR, by reinforcing the kinetic preference of RavS to phosphorylate RavR. [c-di-GMP]-RavS binding effectively decreases the phosphorylation level of RavS and negatively regulates bacterial swimming. Intriguingly, the EAL domain of RavR counteracts the above regulation by degrading c-di-GMP and then increasing the level of phosphorylated RavS. Therefore, RavR acts as a bifunctional phosphate sink that finely controls the level of phosphorylated RavS. These biochemical processes interactively modulate the phosphoryl flux between RavS-RavR and bacterial lifestyle transition. Our results revealed that c-di-GMP acts as an allosteric effector to dynamically modulate specificity between HK and RR.
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