ArticlePloS one2019
Detection of Murine Astrovirus and Myocoptes musculinus in individually ventilated caging systems: Investigations to expose suitable detection methods for routine hygienic monitoring.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.
- Pooled it
- Effect of temperature, relative humidity, and time on the detection of swine RNA viruses (PRRSV, PEDV, IAV) inoculated onto filter papers.Frontiers in cellular and infection microbiology · 2026Article
- Institutional Cost Savings and Improved Pathogen Detection by Replacing Sentinel Mice with Environmental Soiled Bedding Health Monitoring Methods.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Evaluation of Direct Colony Sampling Compared with Sentinel-Free Soiled Bedding Testing for Murine Quarantine Programs.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- Rodent Pathogen Detection via Testing of Soiled Nesting Material.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- A survey on the implementation of environmental health monitoring in mouse facilities in German-speaking countries.PloS one · 2025Article
- Pathogen Prevalence Estimates and Diagnostic Methodology Trends in Laboratory Mice and Rats from 2003 to 2020.Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- A Cross-sectional Survey on Rodent Environmental Health Monitoring Practices: Benchmarking, Associations, and Barriers.Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- Microbiota and environmental health monitoring of mouse colonies by metagenomic shotgun sequencing.World journal of microbiology & biotechnology · 2022Article
- Using Filter Media and Soiled Bedding in Disposable Individually Ventilated Cages as a Refinement to Specific Pathogen-free Mouse Health Monitoring Programs.Journal of the American Association for Laboratory Animal Science : JAALAS · 2022Article
- Identification and Control of anComparative medicine · 2022Article
- Health Monitoring of Laboratory Rodent Colonies-Talking about (R)evolution.Animals : an open access journal from MDPI · 2021Review
- PCR Testing of Media Placed in Soiled Bedding as a Method for Mouse Colony Health Surveillance.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- Evaluation of In-cage Filter Paper as a Replacement for Sentinel Mice in the Detection of Murine Pathogens.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- Cost Comparison of Rodent Soiled Bedding Sentinel and Exhaust Air Dust Health-Monitoring Programs.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- PCR and RT-PCR in the Diagnosis of Laboratory Animal Infections and in Health Monitoring.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
- Detection of Lactate Dehydrogenase Elevating Virus in a Mouse Vivarium Using an Exhaust Air Dust Health Monitoring Program.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Murine Astrovirus is one of the most prevalent viral agents in laboratory rodent facilities worldwide, but its influence on biomedical research results is poorly examined. Due to possible influence on research results and high seroprevalence rates in mice, it appears useful to include this virus into routine health monitoring programs. In order to establish exhaust air particle PCR as a reliable detection method for Murine Astrovirus infections in mice kept in individually ventilated cages (IVC) and compare the method to sentinel mice monitoring regarding reproducibility and detection limit, we conducted a study with defined Murine Astrovirus cage prevalence. In parallel, the efficacy of both detection strategies (soiled-bedding sentinel (SBS) and exhaust air dust (EAD) analysis) was tested for Myocoptes musculinus. The fur mite was used as a reference organism during the whole study period to ensure the validity of this method. Because some publications already demonstrated successful detection of several pathogens, including murine fur mite species, via EAP-PCR. Detection of Murine Astrovirus infections at low prevalence is possible with both methods tested. Detection by exhaust air particles (EAP) is faster, more sensitive and more reliable compared to soiled bedding sentinels (SBS). Exhaust air particle PCR also detected the reference organism Myocoptes musculinus, which was not detected at all by sentinel mice, not even by high sensitivity fur swab qPCR. In conclusion, Murine Astrovirus can be detected by both exhaust air particle PCR and soiled bedding sentinels. We recommend exhaust air particle PCR as the better detection technique for Murine Astrovirus, because it is more reliable. Environmental samples are the method of choice for detection of Myocoptes musculinus because relying on soiled bedding sentinels harbors a big risk of missing existing infestations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.