Evidence map›Paper›PMID 31408166›Full record

ArticleCerebral cortex (New York, N.Y. : 1991)2020

Cadmium versus Lanthanum Effects on Spontaneous Electrical Activity and Expression of Connexin Isoforms Cx26, Cx36, and Cx45 in the Human Fetal Cortex.

Dusica M Kocovic, Pallavi V Limaye, Lauren C H Colburn, Mandakini B Singh, Milena M Milosevic, Jasmina Tadic, Milos Petronijevic, Svetlana Vrzic-Petronijevic, Pavle R Andjus, Srdjan D Antic

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cerebral cortex (New York, N.Y. : 1991), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. COInternational journal of molecular sciences · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Dusica M KocovicFaculty of Biology, University of Belgrade, Belgrade 11000, Serbia.
Pallavi V LimayeInstitute for Systems Genomics, Stem Cell Institute, Department of Neuroscience, UConn Health, Farmington, CT 06030, USA.
Lauren C H ColburnInstitute for Systems Genomics, Stem Cell Institute, Department of Neuroscience, UConn Health, Farmington, CT 06030, USA.
Mandakini B SinghInstitute for Systems Genomics, Stem Cell Institute, Department of Neuroscience, UConn Health, Farmington, CT 06030, USA.
Milena M MilosevicFaculty of Biology, University of Belgrade, Belgrade 11000, Serbia.
Jasmina TadicFaculty of Medicine, University of Belgrade, Belgrade 11000, Serbia.
Milos PetronijevicFaculty of Medicine, University of Belgrade, Belgrade 11000, Serbia.
Svetlana Vrzic-PetronijevicFaculty of Medicine, University of Belgrade, Belgrade 11000, Serbia.
Pavle R AndjusFaculty of Biology, University of Belgrade, Belgrade 11000, Serbia.
Srdjan D AnticInstitute for Systems Genomics, Stem Cell Institute, Department of Neuroscience, UConn Health, Farmington, CT 06030, USA.
University of Belgrade · RSNeural Stem Cell Institute · US

Funding

Sparse, Strong and Large Area Targeting of Genetically Encoded IndicatorsU01MH109091 · NIMH · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI ANTIC, SRDJAN D, KNOPFEL, THOMAS · 2015 to 2017
$1.6M
Connexin Channels in Human NeuronsR21MH104775 · NIMH · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI ANTIC, SRDJAN D · 2016 to 2017
$432k
NIMH NIH HHS R21 MH104775NIMH NIH HHS U01 MH109091
6 · The paper itself

Abstract

Electrical activity is important for brain development. In brain slices, human subplate neurons exhibit spontaneous electrical activity that is highly sensitive to lanthanum. Based on the results of pharmacological experiments in human fetal tissue, we hypothesized that hemichannel-forming connexin (Cx) isoforms 26, 36, and 45 would be expressed on neurons in the subplate (SP) zone. RNA sequencing of dissected human cortical mantles at ages of 17-23 gestational weeks revealed that Cx45 has the highest expression, followed by Cx36 and Cx26. The levels of Cx and pannexin expression between male and female fetal cortices were not significantly different. Immunohistochemical analysis detected Cx45- and Cx26-expressing neurons in the upper segment of the SP zone. Cx45 was present on the cell bodies of human SP neurons, while Cx26 was found on both cell bodies and dendrites. Cx45, Cx36, and Cx26 were strongly expressed in the cortical plate, where newborn migrating neurons line up to form cortical layers. New information about the expression of 3 "neuronal" Cx isoforms in each cortical layer/zone (e.g., SP, cortical plate) and pharmacological data with cadmium and lanthanum may improve our understanding of the cellular mechanisms underlying neuronal development in human fetuses and potential vulnerabilities.

Indexed as

CadmiumCerebral CortexConnexin 26ConnexinsFemaleFetusGap Junction delta-2 ProteinHumansLanthanumMaleMembrane PotentialsNeuronsProtein IsoformsRNA, MessengerCadmiumConnexin 26ConnexinsGap Junction delta-2 ProteinGJB2 protein, humanLanthanumProtein IsoformsRNA, Messengercortical plateNurr1pannexinsubplate zonesubventricular zone

Identifiers

PMID31408166
PMCPMC7132928
OpenAlexW2967773021

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.