ArticleMethods in molecular biology (Clifton, N.J.)2019
Programmable Chromosome Painting with Oligopaints.
Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Drosophila Rumpelstiltskin regulates Polycomb cis and trans interactions and Homie barrier activity.Epigenetics & chromatin · 2026Article
- Genome-wide crossover analysis in the holocentric pantry moth reveals strong crossover assurance and interference during spermatogenesis.Genome research · 2026Article
- Combining ultrastructure expansion microscopy with immunofluorescence and Oligopaint DNA FISH.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Article
- Inferring chromatin architecture at a single locus through probabilistic in situ DNA localization.Nature communications · 2026Article
- Intrinsic mechanotransduction during apical constriction licenses lineage competence in pluripotent stem cells.bioRxiv : the preprint server for biology · 2025Article
- CTCF/RAD21 organize the ground state of chromatin-nuclear speckle association.Nature structural & molecular biology · 2025Article
- B cell stimulation changes the structure and higher-order organization of the inactive X chromosome.Cell reports · 2025Article
- Nuclear speckles regulate functional programs in cancer.Nature cell biology · 2025Article
- Whole Mount Oligopaint Fluorescence In Situ Hybridization of Mouse Oocytes, Eggs, or Early-Stage Embryos.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Inter-chromosomal contacts demarcate genome topology along a spatial gradient.Nature communications · 2024Article
- Chromosome segregation during spermatogenesis occurs through a unique center-kinetic mechanism in holocentric moth species.PLoS genetics · 2024Article
- A feedback loop between heterochromatin and the nucleopore complex controls germ-cell-to-oocyte transition during Drosophila oogenesis.Developmental cell · 2023Article
- Cytogenetic screening of a Canadian swine breeding nucleus using a newly developed karyotyping method named oligo-banding.Genetics, selection, evolution : GSE · 2023Article
- Chromosome-level assembly of the Rangifer tarandus genome and validation of cervid and bovid evolution insights.BMC genomics · 2023Article
- Nucleoporins facilitate ORC loading onto chromatin.Cell reports · 2022Article
- Dosage compensation inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity.Nature communications · 2021Article
- Oligopaint DNA FISH reveals telomere-based meiotic pairing dynamics in the silkworm, Bombyx mori.PLoS genetics · 2021Article
- p53 mediates target gene association with nuclear speckles for amplified RNA expression.Molecular cell · 2021Article
- Core Components of the Nuclear Pore Bind Distinct States of Chromatin and Contribute to Polycomb Repression.Molecular cell · 2020Article
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2 authors.
Funding
Abstract
Current methods for chromosome painting via fluorescence in situ hybridization (FISH) are costly, time-consuming, and limited in complexity. In contrast to conventional sources of probe, Oligopaints are computationally designed, synthesized on microarrays, and amplified by PCR. This approach allows for precise control over the sequences they target, which can range from a few kilobases to entire chromosomes with the same basic protocol. We have utilized the flexibility and scalability of Oligopaints to generate low-cost and renewable chromosome paints for Drosophila, mouse, and human chromosomes. These Oligopaint libraries can be customized to label any genomic feature(s) in a chromosome-wide manner. Additionally, this method is compatible with sequential FISH to label entire genomes with a single denaturation step. Here, we outline a protocol and considerations to scale the Oligopaint technology for fluorescent labeling of whole chromosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.