ArticleAntonie van Leeuwenhoek2019
New genus-specific primers for PCR identification of Rubrobacter strains.
Article in Antonie van Leeuwenhoek, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Impact of mineral fertilization andFrontiers in microbiology · 2025Article
- The Distribution and Influencing Factors of Hypolithic Microbial Communities in the Hexi Corridor.Microorganisms · 2023Article
- Generation of a high quality library of bioactive filamentous actinomycetes from extreme biomes using a culture-based bioprospecting strategy.Frontiers in microbiology · 2022Article
- The First Insight into Polyhydroxyalkanoates Accumulation in Multi-ExtremophilicMicroorganisms · 2021Article
- Diversity and Distribution of Uncultured and Cultured Gaiellales and Rubrobacterales in South China Sea Sediments.Frontiers in microbiology · 2021Article
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Authors and funding
6 authors.
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Abstract
A set of oligonucleotide primers, Rubro223f and Rubro454r, were found to amplify a 267 nucleotide sequence of 16S rRNA genes of Rubrobacter type strains. The primers distinguished members of this genus from other deeply-rooted actinobacterial lineages corresponding to the genera Conexibacter, Gaiella, Parviterribacter, Patulibacter, Solirubrobacter and Thermoleophilum of the class Thermoleophilia. Amplification of DNA bands of about 267 nucleotides were generated from environmental DNA extracted from soil samples taken from two locations in the Atacama Desert. Sequencing of a DNA library prepared from the bands showed that all of the clones fell within the evolutionary radiation occupied by the genus Rubrobacter. Most of the clones were assigned to two lineages that were well separated from phyletic lines composed of Rubrobacter type strains. It can be concluded that primers Rubro223f and Rubro454r are specific for the genus Rubrobacter and can be used to detect the presence and abundance of members of this genus in the Atacama Desert and other biomes.
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