Evidence map›Paper›PMID 31406397›Full record

ReviewJournal of parasitic diseases : official organ of the Indian Society for Parasitology2019

Comparison between different methods of DNA isolation from dried blood spots for determination of malaria to determine specificity and cost effectiveness.

Barsa Baisalini Panda, Arup Shankar Meher, Rupenangshu Kumar Hazra

Abstract readReview
In one paragraph

Review in Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
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  15. Development of a Simple DNA Extraction Method andPathogens (Basel, Switzerland) · 2022
    Article
  16. Article
  17. Preservation and Extraction of Malaria Parasite DNA from Dried Blood Spots.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Barsa Baisalini Panda1Division of Medical Entomology, Regional Medical Research Centre, Indian Council of Medical Research, Nalco Square, Chandrasekharpur, Bhubaneswar, Odisha 751023 India.ORCID 0000-0001-8681-5822
Arup Shankar Meher1Division of Medical Entomology, Regional Medical Research Centre, Indian Council of Medical Research, Nalco Square, Chandrasekharpur, Bhubaneswar, Odisha 751023 India.
Rupenangshu Kumar Hazra1Division of Medical Entomology, Regional Medical Research Centre, Indian Council of Medical Research, Nalco Square, Chandrasekharpur, Bhubaneswar, Odisha 751023 India.ORCID 0000-0002-1773-0005

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA extraction from filter paper by using different methods was compiled through a thorough review of many research articles published in various journals. When performing malaria epidemiological surveys in remote area, it is difficult to collect blood samples and transport it. In field particularly in remote area where facilities for storing and processing of samples does not exist, there surveillance and diagnosis of malaria is very difficult. In this review we are focused upon four simple methods of DNA isolation from the field collected blood and mosquito abdomen blood meal spotted on Whatman No. 1 or No. 3 filter paper. The main DNA isolation methods are Chelex-100, Tris-EDTA (TE) buffer; Methanol based DNA extraction and Phosphate buffer saline (PBS) using Lysis buffer and Phenol-Chloroform method. Efforts have been taken to identify the methods which are cost-effective and take less time to extract DNA from dried blood spots (DBS) and whole mosquitoes. The purpose of this paper is to update the knowledge and find a method to extract DNA from DBS which will be specific, rapid, cost-effective, less time consuming and feasible for epidemiological survey in remote area.

Indexed as

Chelex-100ExtractionMethanolPBSTE-Buffer

Identifiers

PMID31406397
PMCPMC6667532

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.