ArticleScientific reports2019
A universal approach to investigate circRNA protein coding function.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Blood-based epigenetic biomarkers in rheumatoid arthritis: current knowledge and future perspectives.Frontiers in immunology · 2026Review
- Unveiling the Role of circRNAs in Pyroptotic Signalling: From Molecular Crosstalk to Disease Modulation.Journal of cellular and molecular medicine · 2025Review
- Translation of circular RNAs.Nucleic acids research · 2025Review
- Circular RNA-Encoded Proteins in Disease Pathogenesis.International journal of biological sciences · 2025Review
- Molecular mechanisms of circular RNA translation.Experimental & molecular medicine · 2024Review
- Circular RNAs: New layer of complexity evading breast cancer heterogeneity.Non-coding RNA research · 2023Article
- Circular RNA circ-BNC2 (hsa_circ_0008732) inhibits the progression of ovarian cancer through microRNA-223-3p/ FBXW7 axis.Journal of ovarian research · 2022Article
- Profile ofInternational journal of molecular sciences · 2022Article
- CircPrimer 2.0: a software for annotating circRNAs and predicting translation potential of circRNAs.BMC bioinformatics · 2022Article
- CRAFT: a bioinformatics software for custom prediction of circular RNA functions.Briefings in bioinformatics · 2022Article
- CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein.Molecular cancer · 2022Article
- Insights into circular RNAs: their biogenesis, detection, and emerging role in cardiovascular disease.RNA biology · 2021Review
- The design and synthesis of circular RNAs.Methods (San Diego, Calif.) · 2021Review
- Organellar Introns in Fungi, Algae, and Plants.Cells · 2021Review
- On the functional relevance of spatiotemporally-specific patterns of experience-dependent long noncoding RNA expression in the brain.RNA biology · 2021Review
- Rolling-translated EGFR variants sustain EGFR signaling and promote glioblastoma tumorigenicity.Neuro-oncology · 2021Article
- Strategy for Scanning Peptide-Coding Circular RNAs in Colorectal Cancer Based on Bioinformatics Analysis and Experimental Assays.Frontiers in cell and developmental biology · 2021Article
- Circular RNAs: Novel Players in the Oxidative Stress-Mediated Pathologies, Biomarkers, and Therapeutic Targets.Oxidative medicine and cellular longevity · 2021Review
- Large-scale circular RNA deregulation in T-ALL: unlocking unique ectopic expression of molecular subtypes.Blood advances · 2020Article
- The new function of circRNA: translation.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2020Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circular RNAs (circRNAs) are an emerging class of RNA molecules that have been linked to human diseases and important regulatory pathways. Their functional roles are still under investigation, often hampered by inefficient circRNA formation in and ex vivo. We generated an intron-mediated enhancement (IME) system that-in comparison to previously published methods-increases circRNA formation up to 5-fold. This strategy also revealed previously undetected translation of circRNA, e.g., circRtn4. Substantiated by Western blots and mass spectrometry we showed that in mammalian cells, translation of circRtn4 containing a potential "infinite" circular reading frame resulted in "monomers" and extended proteins, presumably "multimer" tandem repeats. In order to achieve high levels of circRNA formation and translation of other natural or recombinant circRNAs, we constructed a versatile circRNA expression vector-pCircRNA-DMo. We demonstrated the general applicability of this method by efficiently generating two additional circRNAs exhibiting high expression levels. The circRNA expression vector will be an important tool to investigate different aspects of circRNA biogenesis and to gain insights into mechanisms of circular RNA translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.