ArticleScientific reports2019
Reproducibility via coordinated standardization: a multi-center study in a Shank2 genetic rat model for Autism Spectrum Disorders.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 2 syntheses or guidelines pooled it, 32 citations in OpenAlex.
- A systematic review of the development and application of home cage monitoring in laboratory mice and rats.BMC biology · 2023Pooled it
- Pooled it
- Data Analysis Planning and Reporting for Confirmatory Multi-Lab Preclinical Trials: A Tutorial.Biometrical journal. Biometrische Zeitschrift · 2026Review
- A scoping review on metrics to quantify reproducibility: a multitude of questions leads to a multitude of metrics.Royal Society open science · 2025Review
- Open science interventions to improve reproducibility and replicability of research: a scoping review.Royal Society open science · 2025Article
- Article
- Distinct patterns of gene expression in the medial preoptic area are related to gregarious singing behavior in European starlings (Sturnus vulgaris).BMC neuroscience · 2023Article
- Blueprints for measuring natural behavior.iScience · 2022Review
- Role of Environment and Experimenter in Reproducibility of Behavioral Studies With Laboratory Mice.Frontiers in behavioral neuroscience · 2022Article
- Genomic Strategies for Understanding the Pathophysiology of Autism Spectrum Disorder.Frontiers in molecular neuroscience · 2022Review
- Using touchscreen-delivered cognitive assessments to address the principles of the 3Rs in behavioral sciences.Lab animal · 2021Review
- Measuring Behavior in the Home Cage: Study Design, Applications, Challenges, and Perspectives.Frontiers in behavioral neuroscience · 2021Article
- Aquarium: open-source laboratory software for design, execution and data management.Synthetic biology (Oxford, England) · 2021Article
- Three Pillars of Automated Home-Cage Phenotyping of Mice: Novel Findings, Refinement, and Reproducibility Based on Literature and Experience.Frontiers in behavioral neuroscience · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 7 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inconsistent findings between laboratories are hampering scientific progress and are of increasing public concern. Differences in laboratory environment is a known factor contributing to poor reproducibility of findings between research sites, and well-controlled multisite efforts are an important next step to identify the relevant factors needed to reduce variation in study outcome between laboratories. Through harmonization of apparatus, test protocol, and aligned and non-aligned environmental variables, the present study shows that behavioral pharmacological responses in Shank2 knockout (KO) rats, a model of synaptic dysfunction relevant to autism spectrum disorders, were highly replicable across three research centers. All three sites reliably observed a hyperactive and repetitive behavioral phenotype in KO rats compared to their wild-type littermates as well as a dose-dependent phenotype attenuation following acute injections of a selective mGluR1 antagonist. These results show that reproducibility in preclinical studies can be obtained and emphasizes the need for high quality and rigorous methodologies in scientific research. Considering the observed external validity, the present study also suggests mGluR1 as potential target for the treatment of autism spectrum disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.