ArticleMicrobial cell factories2019
Drug metabolite synthesis by immobilized human FMO3 and whole cell catalysts.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 16 citations in OpenAlex.
- Immobilised-enzyme microreactors for the identification and synthesis of conjugated drug metabolites.RSC advances · 2023Article
- Bioconversion of vitamin DBiotechnology for biofuels and bioproducts · 2022Review
- Expression of Concern to: Drug metabolite synthesis by immobilized human FMO3 and whole cell catalysts.Microbial cell factories · 2020Article
Corrections and comments
- Expression of concern · 2020-03-25Lack of Approval from Company/Institution ·
- Expression of concern
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSufficient reference standards of drug metabolites are required in the drug discovery and development process. However, such drug standards are often expensive or not commercially available. Chemical synthesis of drug metabolite is often difficulty due to the highly regio- and stereo-chemically demanding. The present work aims to construct stable and efficient biocatalysts for the generation of drug metabolites in vitro.
resultIn this work, using benzydamine as a model drug, two easy-to-perform approaches (whole cell catalysis and enzyme immobilization) were investigated for the synthesis of FMO3-generated drug metabolites. The whole cell catalysis was carried out by using cell suspensions of E. coli JM109 harboring FMO3 and E. coli BL21 harboring GDH (glucose dehydrogenase), giving 1.2 g/L benzydamine N-oxide within 9 h under the optimized conditions. While for another approach, two HisTrap HP columns respectively carrying His
conclusionsThe results presented in this work provides new possibilities for the drug-metabolizing enzymes-mediated biocatalysis.
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