ArticleJournal of experimental botany2019
Barley cysteine protease PAP14 plays a role in degradation of chloroplast proteins.
Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Activity-Based Profiling of Papain-like Cysteine Proteases in Different Plant Organs During Barley Development.Plants (Basel, Switzerland) · 2026Article
- PtCP1 Is an Extraplastidial Cysteine Protease Involved in Leaf Protein Degradation ofPlants (Basel, Switzerland) · 2026Article
- Activity-Based Profiling of Papain-like Cysteine Proteases During Late-Stage Leaf Senescence in Barley.Plants (Basel, Switzerland) · 2025Article
- Maize leaf yellowing gene ZmCAAX modulates growth and drought resistance by regulating abscisic acid contents through interaction with the ABA biosynthetic enzyme ZmNCED3.Plant biotechnology journal · 2025Article
- Comparative physiological and cytological analysis, and omics approach provide clues to the coloring mechanism of the pumpkin yellow stems.BMC plant biology · 2025Article
- Cathepsin B- and L-like Protease Activities Are Induced During Developmental Barley Leaf Senescence.Plants (Basel, Switzerland) · 2024Article
- Identifying aPeerJ · 2024Article
- Maquiberry Cystatins: Recombinant Expression, Characterization, and Use to Protect Tooth Dentin and Enamel.Biomedicines · 2023Article
- Transfection of Barley Leaf Protoplasts with a Fluorescently Tagged Fungal Effector for In Planta Localization Studies in Barley.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Excess nitrogen responsive HvMADS27 transcription factor controls barley root architecture by regulating abscisic acid level.Frontiers in plant science · 2022Article
- Chloroplast dismantling in leaf senescence.Journal of experimental botany · 2021Review
- Leaf senescence: progression, regulation, and application.Molecular horticulture · 2021Review
- Papain-like cysteine proteases are required for the regulation of photosynthetic gene expression and acclimation to high light stress.Journal of experimental botany · 2021Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplast protein degradation is known to occur both inside chloroplasts and in the vacuole. Genes encoding cysteine proteases have been found to be highly expressed during leaf senescence. However, it remains unclear where they participate in chloroplast protein degradation. In this study HvPAP14, which belongs to the C1A family of cysteine proteases, was identified in senescing barley (Hordeum vulgare L.) leaves by affinity enrichment using the mechanism-based probe DCG-04 targeting cysteine proteases and subsequent mass spectrometry. Biochemical analyses and expression of a HvPAP14:RFP fusion construct in barley protoplasts was used to identify the subcellular localization and putative substrates of HvPAP14. The HvPAP14:RFP fusion protein was detected in the endoplasmic reticulum and in vesicular bodies. Immunological studies showed that HvPAP14 was mainly located in chloroplasts, where it was found in tight association with thylakoid membranes. The recombinant enzyme was activated by low pH, in accordance with the detection of HvPAP14 in the thylakoid lumen. Overexpression of HvPAP14 in barley revealed that the protease can cleave LHCB proteins and PSBO as well as the large subunit of Rubisco. HvPAP14 is involved in the normal turnover of chloroplast proteins and may have a function in bulk protein degradation during leaf senescence.
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