Evidence map›Paper›PMID 31403627›Full record

ArticleJournal of visualized experiments : JoVE2019

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription.

Malhar Desai, Rong Di, Huizhou Fan

Abstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Silkworm and spider silk electrospinning: a review.Environmental chemistry letters · 2021
    Review
  12. Tylosin InhibitsFrontiers in veterinary science · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Malhar DesaiDepartment of Pharmacology, Robert Wood Johnson Medical School, Rutgers University; Graduate Program in Physiology and Integrative Biology, School of Graduate Studies, Rutgers University.
Rong DiDepartment of Plant Biology, School of Environmental and Biological, Rutgers University.
Huizhou FanDepartment of Pharmacology, Robert Wood Johnson Medical School, Rutgers University; Graduate Program in Physiology and Integrative Biology, School of Graduate Studies, Rutgers University; fanhu@rutgers.edu.

Funding

GrgA:a Multifunctional Transcription Factor that Control Chlamydial Gene ExpressionR21AI140167 · NIAID · RBHS-ROBERT WOOD JOHNSON MEDICAL SCHOOL · PI FAN, HUIZHOU · 2019 to 2020
$435k
GrgA: Key Regulator of Chlamydial Physiology and potential Antichlamydial TargetR21AI122034 · NIAID · RBHS-ROBERT WOOD JOHNSON MEDICAL SCHOOL · PI FAN, HUIZHOU · 2016 to 2017
$414k
NIAID NIH HHS R21 AI122034NIAID NIH HHS R21 AI140167
6 · The paper itself

Abstract

A transcription factor (TF) is a protein that regulates gene expression by interacting with the RNA polymerase, another TF, and/or template DNA. GrgA is a novel transcription activator found specifically in the obligate intracellular bacterial pathogen Chlamydia. Protein pulldown assays using affinity beads have revealed that GrgA binds two σ factors, namely σ

Indexed as

Transcription, GeneticBacterial ProteinsChlamydiaDNA-Directed RNA PolymerasesGene Expression Regulation, BacterialInterferometryKineticsPromoter Regions, GeneticProtein BindingSigma FactorTranscription FactorsBacterial ProteinsDNA-Directed RNA PolymerasesSigma FactorTranscription Factors

Identifiers

PMID31403627
PMCPMC6693641

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.