Evidence map›Paper›PMID 31402398›Full record

ArticleClinical oral investigations2020

Detection of HPV in oral leukoplakia by brushing and biopsy: prospective study in an Italian cohort.

Fedora Della Vella, Giuseppe Pannone, Assunta Patano, Rossella Ninivaggi, Raffaele Del Prete, Dorina Lauritano, Massimo Petruzzi

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Clinical oral investigations, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

  1. Observational
  2. Review
  3. Review
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Fedora Della VellaInterdisciplinary Department of Medicine, University of Bari "Aldo Moro", Bari, Italy. dellavellaf@gmail.com.ORCID http://orcid.org/0000-0002-6467-2039
Giuseppe PannoneDepartment of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.
Assunta PatanoInterdisciplinary Department of Medicine, University of Bari "Aldo Moro", Bari, Italy.
Rossella NinivaggiInterdisciplinary Department of Medicine, University of Bari "Aldo Moro", Bari, Italy.
Raffaele Del PreteInterdisciplinary Department of Medicine, University of Bari "Aldo Moro", Bari, Italy.
Dorina LauritanoDepartment of Medicine and Surgery, |University of Milan-Bicocca, Monza, Italy.
Massimo PetruzziInterdisciplinary Department of Medicine, University of Bari "Aldo Moro", Bari, Italy. massimo.petruzzi@uniba.it.
University of Bari Aldo Moro · ITUniversity of Foggia · ITUniversity of Milano-Bicocca · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimThe aim of the present study was to evaluate the prevalence of HPV infection in oral leukoplakia, specifying the HPV genotypes eventually involved. We also compared the micro-biopsy and brushing HPV detecting efficacy. MATERIALS AND

methodsConsecutive patients with a presumptive diagnosis of oral leukoplakia were enrolled. Demographical, behavioral data (smoking, alcohol) and lesion features were recorded. Each patient underwent a brushing procedure, performed with a cytobrush rubbed on the lesion, and then a biopsy was performed. The brushing and micro-biopsy specimens were both analyzed with the HPV 28 Anyplex II Seegene RT-PCR. The prevalence of HPV infection was calculated considering the two methods' outcomes separately and then combining both. Cohen's k coefficient was used to assess the agreement between the two methods.

resultsSixty-five patients were enrolled with a mean age of 60 years. The HPV infection prevalence was 17%, decreasing to 5% considering the brushing outcomes alone. The most frequently detected genotypes were 6 (12%), 11 (3%), 42 (3%), and 16 (3%). No statistically significant correlation was found between HPV infection and the variables analyzed, except for smoking and the type of mucosa (p < 0.05). The strength of agreement between cytobrush and micro-biopsy was "fair" (k = 0.384).

conclusionsThe present study showed a low prevalence of HPV infection in oral leukoplakia. The micro-biopsy appeared to be more reliable than brushing in detecting HPV DNA in oral leukoplakia, but the method invasiveness discourages its employ as a screening tool. The importance of HPV in the etiopathogenesis of oral potentially malignant lesions remains unclear; further studies are needed to establish the HPV role in oral leukoplakia. CLINICAL RELEVANCE: HPV involvement in oral leukoplakia and an effective and appropriate detecting technique are still a debated issue. From this study, the restricted use of brushing did not appear sufficient to assess the presence of HPV infection with PCR techniques in samples obtained from oral leukoplakia.

Indexed as

BiopsyHumansItalyLeukoplakia, OralMiddle AgedPapillomavirus InfectionsProspective StudiesBrushingHPVLeukoplakiaOral premalignant disorderPapilloma virusRT PCR

Identifiers

PMID31402398
OpenAlexW2968316326

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.