ArticleStem cell reports2019
Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells.
Article in Stem cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed.
- Depletion of Sorcs3 Activates Totipotency in Mouse Embryonic Stem Cells by Modulating Key Signaling Pathways.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Efficient stem cell-derived mouse embryo models for environmental studies.Developmental cell · 2026Article
- The building blocks of embryo models: embryonic and extraembryonic stem cells.Cell discovery · 2025Review
- Exploring the impacts of senescence on implantation and early embryonic development using totipotent cell-derived blastoids.Journal of advanced research · 2025Article
- Capture of Totipotency in Mouse Embryonic Stem Cells in the Absence of Pdzk1.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Pig blastocyst-like structure models from embryonic stem cells.Cell discovery · 2024Article
- Article
- Blastocyst-like Structures in the Peripheral Retina of Young Adult Beagles.International journal of molecular sciences · 2024Article
- A comprehensive review: synergizing stem cell and embryonic development knowledge in mouse and human integrated stem cell-based embryo models.Frontiers in cell and developmental biology · 2024Review
- Haploid-genetic screening of trophectoderm specification identifiesScience advances · 2023Article
- Modelling in vitro gametogenesis using induced pluripotent stem cells: a review.Cell regeneration (London, England) · 2023Review
- In Vitro Embryogenesis and Gastrulation Using Stem Cells in Mice and Humans.International journal of molecular sciences · 2023Review
- Shifting early embryology paradigms: Applications of stem cell-based embryo models in bioengineering.Current opinion in genetics & development · 2023Review
- A chemically defined system supports two distinct types of stem cell from a single blastocyst and their self-assembly to generate blastoid.Cell proliferation · 2023Article
- Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm.Protein & cell · 2023Article
- Stem cell-based models of early mammalian development.Development (Cambridge, England) · 2022Article
- Organoid research on human early development and beyond.Medical review (2021) · 2022Review
- Three-dimensional culture models of human endometrium for studying trophoblast-endometrium interaction during implantation.Reproductive biology and endocrinology : RB&E · 2022Review
- The role of BMP4 signaling in trophoblast emergence from pluripotency.Cellular and molecular life sciences : CMLS · 2022Review
- Efficient cell chatting between embryo and uterus ensures embryo implantation†.Biology of reproduction · 2022Article
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Abstract
Soon after fertilization, the few totipotent cells of mammalian embryos diverge to form a structure called the blastocyst (BC). Although numerous cell types, including germ cells and extended-pluripotency stem cells, have been developed from pluripotent stem cells (PSCs) in vitro, generating functional BCs only from PSCs remains elusive. Here, we describe induced self-organizing 3D BC-like cysts (iBLCs) generated from mouse PSC culture. Resembling natural BCs, iBLCs have a blastocoel-like cavity and were formed with outer cells expressing trophectoderm lineage markers and with inner cells expressing pluripotency markers. iBLCs transplanted to pseudopregnant mice uteruses implanted, induced decidualization, and exhibited growth and development before resorption, demonstrating that iBLCs are implantation competent. iBLC precursor intermediates required the transcription factor Prdm14 and concomitantly activated the totipotency-related cleavage-stage MERVL reporter and 2C genes. Thus, our system may contribute to the understanding of molecular mechanisms underpinning totipotency, embryogenesis, and implantation.
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