ArticleMolecular and cellular probes2019
Establishment of a recombinase polymerase amplification (RPA) assay for the detection of Brucella spp. Infection.
Article in Molecular and cellular probes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Development of a Visual RPA-CRISPR/Cas12a Assay TargetingVeterinary sciences · 2026Article
- Ultrasensitive Detection and Its Potential Applications in the Diagnosis of Brucellosis.Current microbiology · 2026Review
- Development and preliminary validation of anFrontiers in microbiology · 2026Article
- Development of a rapid and sensitive RPA-CRISPR/Cas12a-based assay for the detection ofMicrobiology spectrum · 2025Article
- Rapid detection assays for Bacillus anthracis, Yersinia pestis, and Brucella spp. via triplex-recombinase polymerase amplification.Molecular biology reports · 2025Article
- A recombinase polymerase amplification-SYBR Green I assay for the rapid and visual detection of Brucella.Folia microbiologica · 2024Article
- RPA-CRISPR/Cas12a-LFA combined with a digital visualization instrument to detectMicrobiology spectrum · 2024Article
- Evidence-practice gap analysis in the role of tick in brucellosis transmission: a scoping review.Infectious diseases of poverty · 2024Article
- Recent Advances in Design and Application of Nanomaterials-Based Colorimetric Biosensors for Agri-food Safety Analysis.ACS omega · 2023Review
- Development of an LFD-RPA Assay for Rapid Detection ofCurrent issues in molecular biology · 2023Article
- CRISPR-Cas12a test strip (CRISPR/CAST) package: In-situ detection of Brucella from infected livestock.BMC veterinary research · 2023Article
- Sensitive and rapid detection of Babesia species in dogs by recombinase polymerase amplification with lateral flow dipstick (RPA-LFD).Scientific reports · 2022Article
- Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for ActiveFrontiers in microbiology · 2022Article
- Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications.Frontiers in cellular and infection microbiology · 2022Review
- Bovine brucellosis - a comprehensive review.The veterinary quarterly · 2021Review
- 3D Printed Monolithic Microreactors for Real-Time Detection ofMicromachines · 2020Article
- Specific Detection and Differentiation BetweenFrontiers in veterinary science · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucellosis is a worldwide re-emerging zoonosis. It has an economic impact due to abortion and loss of fertility in livestock. In this study, Real-time recombinase polymerase amplification (RT-RPA-BP26) targeting Brucella spp. bp26 gene and Lateral flow dipstick (LFD-RPA-IS711) combined with SYBR- Green recombinase polymerase amplification (RPA) targeting insertion sequence IS711 region of Brucella spp. bp26 gene, was developed to detect Brucella spp. from different sample types in domestic animals. The sensitivity and specificity of the two developed RPAs were compared with real-time PCR, PCR, and Rose Bengal Plate Test (RBPT). The analytical sensitivity and detection limit of Real-time RPA and LFD RPA were four and six copies per reaction respectively. The detection of six colony forming units (CFU) of the bacteria-bearing construct with the target sequence was within 20 min at 40 °C for Real-time RPA and 37 °C for LFD RPA. The LFD RPA could work at temperatures between 30 and 35 °C and could be completed within 10-30 min. No significant differences were observed when comparing the results from Real-time RPA and LFD RPA to Real-time PCR and PCR. Both methods showed no cross reactivity with Chlamydia abortus, Toxoplasma gondii, Salmonella typhimurium, and Escherichia coli. In conclusion, RPA is a useful and convenient field and point of care test for brucellosis.
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