Evidence map›Paper›PMID 31401280›Full record

ArticleInternational journal of biological macromolecules2019

Simulation-guided enzyme discovery: A new microbial source of cellobiose 2-epimerase.

Yaqin Xiao, Qiuming Chen, Eugene I Shakhnovich, Wenli Zhang, Wanmeng Mu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of biological macromolecules, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 40 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Yaqin XiaoState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Qiuming ChenState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, United States.
Eugene I ShakhnovichDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, United States. Electronic address: shakhnovich@chemistry.harvard.edu.
Wenli ZhangState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address: wenlizhang@jiangnan.edu.cn.
Wanmeng MuState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Food Science and Technology · CNHarvard University · USJiangnan University · CN

Funding

Study of Biological Evolution of Structure and Function in ProteinsR01GM068670 · NIGMS · HARVARD UNIVERSITY · PI SHAKHNOVICH, EUGENE I · 2004 to 2020
$7.0M
NIGMS NIH HHS R01 GM068670
6 · The paper itself

Abstract

Cellobiose 2-epimerase (CE) is a promising industrial enzyme that can be utilized in the dairy industry. More thermostable CEs from different microorganisms are still needed for a higher lactulose productivity. This study demonstrated the feasibility to use molecular dynamics (MD) simulation as the preliminary computational filter for thermostable enzymes screening. Sequence information of eleven uncharacterized CEs were chosen to be analyzed by MD simulations. The CE from Dictyoglomus thermophilum (Dith-CE) was determined experimentally to be one of the most thermostable CEs with the highest epimerization (160 ± 6.5 U mg

Indexed as

Molecular Dynamics SimulationBacteriaCellobioseEnzyme StabilityHydrogen-Ion ConcentrationKineticsLactuloseProtein ConformationRacemases and EpimerasesSequence AlignmentSubstrate SpecificityTemperatureCellobioseLactuloseRacemases and EpimerasesCellobiose 2-epimeraseLactuloseMD simulationThermal stability

Identifiers

PMID31401280
PMCPMC9364981
OpenAlexW2968360702

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.