Evidence map›Paper›PMID 31399606›Full record

ArticleScientific reports2019

Detection of introduced and resident marine species using environmental DNA metabarcoding of sediment and water.

Luke E Holman, Mark de Bruyn, Simon Creer, Gary Carvalho, Julie Robidart, Marc Rius

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
17.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 193 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

Luke E HolmanSchool of Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton, United Kingdom. l.e.holman@soton.ac.uk.ORCID http://orcid.org/0000-0002-8139-3760
Mark de BruynMolecular Ecology and Fisheries Genetics Laboratory, School of Natural Sciences, Bangor University, Bangor, United Kingdom.ORCID http://orcid.org/0000-0003-1528-9604
Simon CreerMolecular Ecology and Fisheries Genetics Laboratory, School of Natural Sciences, Bangor University, Bangor, United Kingdom.ORCID http://orcid.org/0000-0003-3124-3550
Gary CarvalhoMolecular Ecology and Fisheries Genetics Laboratory, School of Natural Sciences, Bangor University, Bangor, United Kingdom.
Julie RobidartOcean Technology and Engineering Group, National Oceanography Centre Southampton, Southampton, United Kingdom.
Marc RiusSchool of Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton, United Kingdom.ORCID http://orcid.org/0000-0002-2195-6605
National Oceanography Centre · GBBangor University · GBThe University of Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Environmental DNA (eDNA) surveys are increasingly being used for biodiversity monitoring, principally because they are sensitive and can provide high resolution community composition data. Despite considerable progress in recent years, eDNA studies examining how different environmental sample types can affect species detectability remain rare. Comparisons of environmental samples are especially important for providing best practice guidance on early detection and subsequent mitigation of non-indigenous species. Here we used eDNA metabarcoding of COI (cytochrome c oxidase subunit I) and 18S (nuclear small subunit ribosomal DNA) genes to compare community composition between sediment and water samples in artificial coastal sites across the United Kingdom. We first detected markedly different communities and a consistently greater number of distinct operational taxonomic units in sediment compared to water. We then compared our eDNA datasets with previously published rapid assessment biodiversity surveys and found excellent concordance among the different survey techniques. Finally, our eDNA surveys detected many non-indigenous species, including several newly introduced species, highlighting the utility of eDNA metabarcoding for both early detection and temporal / spatial monitoring of non-indigenous species. We conclude that careful consideration on environmental sample type is needed when conducting eDNA surveys, especially for studies assessing community change.

Indexed as

AnimalsAquatic OrganismsBiodiversityDNA Barcoding, TaxonomicDNA, EnvironmentalDNA, RibosomalElectron Transport Complex IVEnvironmental MonitoringGeologic SedimentsMetagenomicsUnited KingdomWaterDNA, EnvironmentalDNA, RibosomalElectron Transport Complex IVWater

Identifiers

PMID31399606
PMCPMC6689084
OpenAlexW2966983598

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.