Evidence map›Paper›PMID 31398290›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2019

Surfen-mediated blockade of extratumoral chondroitin sulfate glycosaminoglycans inhibits glioblastoma invasion.

Meghan T Logun, Kallie E Wynens, Gregory Simchick, Wujun Zhao, Leidong Mao, Qun Zhao, Subhas Mukherjee, Daniel J Brat, Lohitash Karumbaiah

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Meghan T LogunRegenerative Bioscience Center, University of Georgia, Athens, Georgia, USA.
Kallie E WynensRegenerative Bioscience Center, University of Georgia, Athens, Georgia, USA.
Gregory SimchickDepartment of Physics and Astronomy, University of Georgia, Athens, Georgia, USA.
Wujun ZhaoDepartment of Chemistry, University of Georgia, Athens, Georgia, USA.
Leidong MaoRegenerative Bioscience Center, University of Georgia, Athens, Georgia, USA.
Qun ZhaoRegenerative Bioscience Center, University of Georgia, Athens, Georgia, USA.
Subhas MukherjeeDepartment of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
Daniel J BratDepartment of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
Lohitash KarumbaiahRegenerative Bioscience Center, University of Georgia, Athens, Georgia, USA.
University of Georgia · USNorthwestern University · US

Funding

Creating Endogenous Stem Cell Niches to Promote Functional Brain Repair Post-TBIR01NS099596 · NINDS · UNIVERSITY OF GEORGIA · PI KARUMBAIAH, LOHITASH · 2017 to 2021
$1.6M
Purchase of an imaging flow cytometer 2016S10OD021719 · OD · UNIVERSITY OF GEORGIA · PI TARLETON, RICK L · 2017 to 2017
$454k
NIH HHS S10 OD021719NINDS NIH HHS R01 NS099596
6 · The paper itself

Abstract

Invasive spread of glioblastoma (GBM) is linked to changes in chondroitin sulfate (CS) proteoglycan (CSPG)-associated sulfated glycosaminoglycans (GAGs) that are selectively up-regulated in the tumor microenvironment (TME). We hypothesized that inhibiting CS-GAG signaling in the TME would stem GBM invasion. Rat F98 GBM cells demonstrated enhanced preferential cell invasion into oversulfated 3-dimensional composite of CS-A and CS-E [4- and 4,6-sulfated CS-GAG (COMP)] matrices compared with monosulfated (4-sulfated) and unsulfated hyaluronic acid matrices in microfluidics-based choice assays, which is likely influenced by differential GAG receptor binding specificities. Both F98 and human patient-derived glioma stem cells (GSCs) demonstrated a high degree of colocalization of the GSC marker CD133 and CSPGs. The small molecule sulfated GAG antagonist bis-2-methyl-4-amino-quinolyl-6-carbamide (surfen) reduced invasion and focal adhesions in F98 cells encapsulated in COMP matrices and blocked CD133 and antichondroitin sulfate antibody (CS-56) detection of respective antigens in F98 cells and human GSCs. Surfen-treated F98 cells down-regulated CSPG-binding receptor transcripts and protein, as well as total and activated ERK and protein kinase B. Lastly, rats induced with frontal lobe tumors and treated with a single intratumoral dose of surfen demonstrated reduced tumor burden and spread compared with untreated controls. These results present a first demonstration of surfen as an inhibitor of sulfated GAG signaling to stem GBM invasion.-Logun, M. T., Wynens, K. E., Simchick, G., Zhao, W., Mao, L., Zhao, Q., Mukherjee, S., Brat, D. J., Karumbaiah, L. Surfen-mediated blockade of extratumoral chondroitin sulfate glycosaminoglycans inhibits glioblastoma invasion.

Indexed as

AC133 AntigenAnimalsCell Line, TumorCell MovementChondroitin SulfatesGlioblastomaGliomaGlycosaminoglycansHumansNeoplasm InvasivenessNeoplastic Stem CellsRatsSignal TransductionTumor MicroenvironmentUreaA73025AC133 Antigenaminoquinuridechondroitin sulfate glycosaminoglycanChondroitin SulfatesGlycosaminoglycansUreaextracellular matrixgliomatumor microenvironment

Identifiers

PMID31398290
PMCPMC6902699
OpenAlexW2967411105

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.