Evidence map›Paper›PMID 31398040›Full record

ArticleJournal of proteome research2019

Comparison of CRISPR Genomic Tagging for Affinity Purification and Endogenous Immunoprecipitation Coupled with Quantitative Mass Spectrometry To Identify the Dynamic AMPKα2 Interactome.

Benjamin D Stein, Sébastien Herzig, Salvador Martínez-Bartolomé, Mathieu Lavallée-Adam, Reuben J Shaw, John R Yates

Open access · greenAbstract readComparative Study
In one paragraph

Article in Journal of proteome research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 8 citations in OpenAlex.

  1. Proteomics-Driven Strategies for Proximity-Inducing Drug Discovery.Angewandte Chemie (International ed. in English) · 2026
    Review
  2. Article
  3. MS-Based Proteomics Approach in Mapping Protein-Protein Interaction.Advances in experimental medicine and biology · 2026
    Review
  4. Expanding the CRISPR/Cas toolkit: applications in proteomics and theranostics.Frontiers in bioengineering and biotechnology · 2025
    Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Benjamin D SteinDepartments of Molecular Medicine and Neurobiology , The Scripps Research Institute , La Jolla , California , United States.
Sébastien HerzigMolecular and Cell Biology Laboratory , The Salk Institute for Biological Studies , La Jolla , California , United States.
Salvador Martínez-BartoloméDepartments of Molecular Medicine and Neurobiology , The Scripps Research Institute , La Jolla , California , United States.ORCID 0000-0001-7592-5612
Mathieu Lavallée-AdamDepartments of Molecular Medicine and Neurobiology , The Scripps Research Institute , La Jolla , California , United States.ORCID 0000-0003-2124-3872
Reuben J ShawMolecular and Cell Biology Laboratory , The Salk Institute for Biological Studies , La Jolla , California , United States.
John R YatesDepartments of Molecular Medicine and Neurobiology , The Scripps Research Institute , La Jolla , California , United States.ORCID 0000-0001-5267-1672
Scripps Research Institute · USSalk Institute for Biological Studies · US

Funding

Viral Vector Core (VVC)P30CA014195 · NCI · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI Ye Zheng · 1985 to 2026
$82.8M
TrainingP41GM103533 · NIGMS · UNIVERSITY OF WASHINGTON · PI MACCOSS, MICHAEL · 2012 to 2021
$20.7M
Decoding And Targeting The LKB1-AMPK Signaling Pathway In CancerR35CA220538 · NCI · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI Reuben Shaw · 2017 to 2026
$11.3M
Proteogenetics of Autism Spectrum DisordersR01MH100175 · NIMH · SCRIPPS RESEARCH INSTITUTE, THE · PI MUOTRI, ALYSSON R., YATES III, JOHN R · 2013 to 2025
$7.4M
Quantitative Proteome Analysis of Brain DevelopmentR01MH067880 · NIMH · SCRIPPS RESEARCH INSTITUTE, THE · PI YATES III, JOHN R · 2003 to 2017
$7.1M
Role of LKB1 and AMPK in Metformin and TZD Control of Glucose Metabolism in LiverR01DK080425 · NIDDK · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI SHAW, REUBEN · 2007 to 2015
$3.6M
AMPK and AMPK-related kinases in lung cancer development and treatmentR01CA172229 · NCI · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI SHAW, REUBEN · 2013 to 2017
$2.0M
NCI NIH HHS P30 CA014195NCI NIH HHS R01 CA172229NCI NIH HHS R35 CA220538NHLBI NIH HHS HHSN268201000035CNIDDK NIH HHS R01 DK080425NIGMS NIH HHS P41 GM103533NIMH NIH HHS R01 MH067880NIMH NIH HHS R01 MH100175
6 · The paper itself

Abstract

Recent advances in genome editing technologies have enabled the insertion of epitope tags at endogenous loci with relative efficiency. We describe an approach for investigation of protein interaction dynamics of the AMP-activated kinase complex AMPK using a catalytic subunit AMPKα2 (

Indexed as

AMP-Activated Protein KinasesAntibodiesCells, CulturedChromatography, AffinityClustered Regularly Interspaced Short Palindromic RepeatsGene EditingGenomicsHEK293 CellsHumansImmunoprecipitationIsotope LabelingMass SpectrometryProtein Interaction MappingAMP-Activated Protein KinasesAntibodiesPRKAA2 protein, humanaffinity purificationAMPKCRISPR taggingdynamic interactionsendogenous immunoprecipitationinteractomicsSILAC

Identifiers

PMID31398040
PMCPMC7512655
OpenAlexW2968398338

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.