ArticleeLife2019
In vivo imaging with a water immersion objective affects brain temperature, blood flow and oxygenation.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Imaging of Neurovascular Activity during Motor Responses Evoked by Displacement-guided Focused Ultrasound (DgFUS) Neuromodulation in Mice.Research square · 2026Article
- Functional ultrasound imaging in preclinical brain stimulation: from multimodal readouts to closed-loop neuromodulation.Frontiers in neuroscience · 2026Review
- Simultaneous thermal and large field-of-view two-photon imaging enables optimization of signal-to-noise.Neurophotonics · 2026Article
- Central cytometabolic functional vascular coupling in health and disease.npj metabolic health and disease · 2025Review
- Brief disruptions in capillary flow result in rapid onset of hypoxia.Neurophotonics · 2025Article
- Article
- MgSOTheranostics · 2025Article
- Quantitative Optical Imaging of Oxygen in Brain Vasculature.The journal of physical chemistry. B · 2024Review
- Analysis of ischemic stroke-mediated effects on blood-brain barrier properties along the arteriovenous axis assessed by intravital two-photon imaging.Fluids and barriers of the CNS · 2024Article
- Numerical study of a convective cooling strategy for increasing safe power levels in two-photon brain imaging.Biomedical optics express · 2024Article
- In vivo methods for imaging blood-brain barrier function and dysfunction.European journal of nuclear medicine and molecular imaging · 2023Review
- Bidirectional alterations in brain temperature profoundly modulate spatiotemporal neurovascular responses in-vivo.Communications biology · 2023Article
- Could respiration-driven blood oxygen changes modulate neural activity?Pflugers Archiv : European journal of physiology · 2023Review
- Neurovascular coupling: motive unknown.Trends in neurosciences · 2022Review
- Baseline oxygen consumption decreases with cortical depth.PLoS biology · 2022Article
- Cranial window for longitudinal and multimodal imaging of the whole mouse cortex.Neurophotonics · 2022Article
- The oxygen initial dip in the brain of anesthetized and awake mice.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Optical measurement of microvascular oxygenation and blood flow responses in awake mouse cortex during functional activation.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2022Article
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Reply to: Rethink the classical view of cerebrospinal fluid production.Nature reviews. Neurology · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Previously, we reported the first oxygen partial pressure (Po2) measurements in the brain of awake mice, by performing two-photon phosphorescence lifetime microscopy at micrometer resolution (Lyons et al., 2016). However, this study disregarded that imaging through a cranial window lowers brain temperature, an effect capable of affecting cerebral blood flow, the properties of the oxygen sensors and thus Po2 measurements. Here, we show that in awake mice chronically implanted with a glass window over a craniotomy or a thinned-skull surface, the postsurgical decrease of brain temperature recovers within a few days. However, upon imaging with a water immersion objective at room temperature, brain temperature decreases by ~2-3°C, causing drops in resting capillary blood flow, capillary Po2, hemoglobin saturation, and tissue Po2. These adverse effects are corrected by heating the immersion objective or avoided by imaging through a dry air objective, thereby revealing the physiological values of brain oxygenation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.