ArticleNucleic acids research2019
Adenovirus major core protein condenses DNA in clusters and bundles, modulating genome release and capsid internal pressure.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Physical Virology with Atomic Force and Fluorescence Microscopies: Seeing and Touching Viruses and Protein Cages.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Viruses under mechanical force: Implications and applications.Virus research · 2026Review
- Using atomic force microscopy for physical virology: touching and manipulating single virus particles.Biophysical reviews · 2026Review
- Development of Three Alternative Strategies for the Binding of Cells to Functionalized DeepTipBiomimetics (Basel, Switzerland) · 2026Article
- Adenovirus maturation establishes the transcription competent packaging of its genome.EMBO reports · 2025Article
- Aviadenovirus structure: A highly thermostable capsid in the absence of stabilizing proteins.PLoS pathogens · 2025Article
- Mysteries of adenovirus packaging.Journal of virology · 2025Review
- Stepwise virus assembly in the cell nucleus revealed by spatiotemporal click chemistry of DNA replication.Science advances · 2024Article
- RETRACTED: Regions of Bovine Adenovirus-3 Protein VII Involved in Interactions with Viral and Cellular Proteins.Viruses · 2024Article
- Identification of Individual Target Molecules Using Antibody-Decorated DeepTipBiomimetics (Basel, Switzerland) · 2024Article
- Atomic Force Microscopy of Viruses.Sub-cellular biochemistry · 2024Review
- Mechanical Properties of Viruses.Sub-cellular biochemistry · 2024Review
- Mechanical tomography of an archaeal lemon-shaped virus reveals membrane-like fluidity of the capsid and liquid nucleoprotein cargo.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Adenovirus protein VII binds the A-box of HMGB1 to repress interferon responses.PLoS pathogens · 2023Article
- Adenovirus core protein V reinforces the capsid and enhances genome release from disrupted particles.Science advances · 2023Article
- Review
- Adenovirus entry: Stability, uncoating, and nuclear import.Molecular microbiology · 2022Review
- Electromechanical Photophysics of GFP Packed Inside Viral Protein Cages Probed by Force-Fluorescence Hybrid Single-Molecule Microscopy.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
- Article
- Review
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
Some viruses package dsDNA together with large amounts of positively charged proteins, thought to help condense the genome inside the capsid with no evidence. Further, this role is not clear because these viruses have typically lower packing fractions than viruses encapsidating naked dsDNA. In addition, it has recently been shown that the major adenovirus condensing protein (polypeptide VII) is dispensable for genome encapsidation. Here, we study the morphology and mechanics of adenovirus particles with (Ad5-wt) and without (Ad5-VII-) protein VII. Ad5-VII- particles are stiffer than Ad5-wt, but DNA-counterions revert this difference, indicating that VII screens repulsive DNA-DNA interactions. Consequently, its absence results in increased internal pressure. The core is slightly more ordered in the absence of VII and diffuses faster out of Ad5-VII- than Ad5-wt fractured particles. In Ad5-wt unpacked cores, dsDNA associates in bundles interspersed with VII-DNA clusters. These results indicate that protein VII condenses the adenovirus genome by combining direct clustering and promotion of bridging by other core proteins. This condensation modulates the virion internal pressure and DNA release from disrupted particles, which could be crucial to keep the genome protected inside the semi-disrupted capsid while traveling to the nuclear pore.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.