Evidence map›Paper›PMID 31396571›Full record

ArticleCommunications biology2019

Amplicon sequencing provides more accurate microbiome information in healthy children compared to culturing.

Shashank Gupta, Martin S Mortensen, Susanne Schjørring, Urvish Trivedi, Gisle Vestergaard, Jakob Stokholm, Hans Bisgaard, Karen A Krogfelt, Søren J Sørensen

Abstract readComparative Study
In one paragraph

Article in Communications biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
74citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

74 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Fungal backpackers-the mycobiome ofFrontiers in microbiology · 2025
    Pooled it
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Gut Microbiota Alterations in Patients With Kawasaki Disease.Arteriosclerosis, thrombosis, and vascular biology · 2025
    Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Review
  20. Article

14 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shashank Gupta *1Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark.
Martin S Mortensen *1Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark.ORCID 0000-0001-5483-7533
Susanne Schjørring2Department of Bacterial, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen S, Denmark.
Urvish Trivedi1Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark.
Gisle Vestergaard1Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark.
Jakob StokholmCopenhagen Prospective Studies on Asthma in Childhood, Faculty of Health Sciences, University of Copenhagen, Copenhagen University Hospital Gentofte, Copenhagen, Denmark.ORCID 0000-0003-4989-9769
Hans BisgaardCopenhagen Prospective Studies on Asthma in Childhood, Faculty of Health Sciences, University of Copenhagen, Copenhagen University Hospital Gentofte, Copenhagen, Denmark.ORCID 0000-0003-4131-7592
Karen A Krogfelt2Department of Bacterial, Parasites and Fungi, Statens Serum Institut, 2300 Copenhagen S, Denmark.ORCID 0000-0001-7536-3453
Søren J Sørensen1Section of Microbiology, Department of Biology, University of Copenhagen, 2100 Copenhagen, Denmark.ORCID 0000-0001-6227-9906

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Next-Generation Sequencing (NGS) of 16S rRNA gene is now one of the most widely used application to investigate the microbiota at any given body site in research. Since NGS is more sensitive than traditional culture methods (TCMs), many studies have argued for them to replace TCMs. However, are we really ready for this transition? Here we compare the diagnostic efficiency of the two methods using a large number of samples (

Indexed as

Bacteriological TechniquesGastrointestinal MicrobiomeHigh-Throughput Nucleotide SequencingRibotypingAge FactorsBacteriaDNA, BacterialFecesFemaleHealthy VolunteersHumansHypopharynxInfantInfant, NewbornMalePhylogenyDNA, BacterialBacteriaMicrobial communities

Identifiers

PMID31396571
PMCPMC6683184

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.