Evidence map›Paper›PMID 31396530›Full record

ArticleBioMed research international2019

Downregulation of miR-224-5p Promotes Migration and Proliferation in Human Dental Pulp Stem Cells.

Zhihong Ke, Zailing Qiu, Tingting Xiao, Jianchai Zeng, Luning Zou, Xuemei Lin, Xuegang Hu, Shihan Lin, Hongbing Lv

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioMed research international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.8field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 24 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Zhihong KeDepartment of Endodontics and Operative Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.
Zailing QiuDepartment of Endodontics and Operative Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.
Tingting XiaoDepartment of Endodontics and Operative Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.
Jianchai ZengDepartment of Endodontics and Operative Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.
Luning ZouDepartment of Endodontics and Operative Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.
Xuemei LinDepartment of Endodontics and Operative Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.
Xuegang HuDepartment of Endodontics and Operative Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.
Shihan LinDepartment of Endodontics and Operative Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.ORCID https://orcid.org/0000-0001-6900-3101
Hongbing LvDepartment of Endodontics and Operative Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350000, China.ORCID https://orcid.org/0000-0002-3855-0097
Fujian Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionPulp regeneration, as a treatment for pulp necrosis, has significant advantages over root canal therapy for the preservation of living pulp. To date, research on pulp regeneration has mainly focused on the transplantation of pulp stem cells into the root canal, but there is still a lack of research on the migration of pulp cells into the root canal via cell homing. Stem cells from the apical tooth papilla (SCAP) are recognized as multidirectional stem cells, but these cells are difficult to obtain. MicroRNAs are small noncoding RNAs that play crucial roles in regulating normal and pathologic functions. We hypothesized that some types of microRNAs might improve the migration and proliferation function of dental pulp stem cells (DPSCs), which are easily obtained in clinical practice, and as a result, DPSCs might replace SCAP and provide valuable information for regenerative endodontics.

methodsMagnetic activated cell sorting of DPSCs and SCAP was performed. Next-generation sequencing was performed to examine DPSCs and SCAP miRNAs expression and to identify the most significant differentially expressed miRNA. CCK-8 and transwell assays were used to determine the impact of this miRNA on DPSCs proliferation and migration.

resultsThe most significant differentially expressed miRNA between DPSCs and SCAP was miR-224-5p. Downregulating miR-224-5p promoted DPSCs proliferation and migration; the opposite results were observed when miR-224-5p was upregulated.

conclusionMiR-224-5p promotes proliferation and migration in DPSCs, a finding that is of great significance for further exploring the role of dental pulp stem cells in regenerative endodontics.

Indexed as

Cell MovementCell ProliferationDown-RegulationAdolescentAdultDental PulpFemaleHumansMaleMicroRNAsStem CellsMicroRNAsMIRN224 microRNA, human

Identifiers

PMID31396530
PMCPMC6668552
OpenAlexW2958384392

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.