ArticleAmerican journal of translational research2019
Sequencing of short cfDNA fragments in NIPT improves fetal fraction with higher maternal BMI and early gestational age.
Article in American journal of translational research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- A Validation Approach for Determining Fetal Blood Groups Non-Invasively by High-Sensitive Next-Generation Sequencing.Journal of clinical medicine · 2025Article
- Characterization of Ultra-Short plasma Cell-Free DNA in maternal blood and its preliminary potential as a screening marker for preeclampsia.Molecular medicine (Cambridge, Mass.) · 2025Article
- High platelet distribution width affects the detection of fetal fraction of cell-free DNA detected by non-invasive prenatal testing.American journal of translational research · 2025Article
- Evaluation of Circulating Tumor DNA as a Liquid Biomarker in Uveal Melanoma.Investigative ophthalmology & visual science · 2024Article
- Insights into non-informative results from non-invasive prenatal screening through gestational age, maternal BMI, and age analyses.PloS one · 2024Article
- Non-Invasive Prenatal Testing (NIPT): Reliability, Challenges, and Future Directions.Diagnostics (Basel, Switzerland) · 2023Review
- Whole genome non-invasive prenatal testing in prenatal screening algorithm: clinical experience from 12,700 pregnancies.BMC pregnancy and childbirth · 2022Article
- A fetal fraction enrichment method reduces false negatives and increases test success rate of fetal chromosome aneuploidy detection in early pregnancy loss.Journal of translational medicine · 2022Article
- Factors Affecting the Fetal Fraction in Noninvasive Prenatal Screening: A Review.Frontiers in pediatrics · 2022Review
- Lipid Metabolism Affects Fetal Fraction and Screen Failures in Non-invasive Prenatal Testing.Frontiers in medicine · 2021Article
- Potential of Next-Generation Sequencing in Noninvasive Fetal Molecular Blood Group Genotyping.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2020Review
- Sequencing Shorter cfDNA Fragments Decreases the False Negative Rate of Non-invasive Prenatal Testing.Frontiers in genetics · 2020Article
- Experimental factors are associated with fetal fraction in size selection noninvasive prenatal testing.American journal of translational research · 2019Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low fetal DNA fraction (< 4%) samples obtained during noninvasive prenatal testing (NIPT) are responsible for 0.5%-3% of "no calls". Maternal characteristics such as body mass index (BMI) and gestational age (GA) are the main factors that influence fetal fraction. Here, we improved fetal fraction by performing semiconductor sequencing of shorter fragments (107-145 bp) of cfDNA (traditional NIPT fragment is 160 bp). Multivariable linear regression modeling was used to evaluate the association between fetal fraction and maternal characteristics including BMI and GA. Among the 1495 shorter cfDNA sequencing samples, BMI and GA were negatively and positively correlated with fetal fraction, respectively. Compared with underweight pregnant women, sequencing of shorter fragments decreased the mean fetal fraction differences between BMI groups, especially in the obese women (~15%). We also showed that the average fetal fraction was 22.2% and 96.3% fetal fraction more than 10% in the obese women with the average GA of 17 weeks. Size selection slightly decreased the mean fetal fraction differences between different GA groups, and sequencing shorter cfDNA can yield a fetal fraction at an earlier GA. Collectively, our results support the strategy of sequencing shorter to improve fetal fraction in subjects with a high maternal BMI and earlier GA.
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Identifiers
31396348PMC6684886W2998066660What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.