Evidence map›Paper›PMID 31396026›Full record

ArticleJournal of applied crystallography2019

Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.

Sylvain Engilberge, Tristan Wagner, Gianluca Santoni, Cécile Breyton, Seigo Shima, Bruno Franzetti, Francois Riobé, Olivier Maury, Eric Girard

Open access · hybridAbstract read
In one paragraph

Article in Journal of applied crystallography, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 35 citations in OpenAlex.

  1. Article
  2. Snapshots ofiScience · 2025
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  7. Article
  8. Multi-crystal data collection using synchrotron radiation as exemplified with low-symmetry crystals of Dps.Acta crystallographica. Section F, Structural biology communications · 2020
    Article
  9. Article
  10. NMR Spectroscopy of supramolecular chemistry on protein surfaces.Beilstein journal of organic chemistry · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Sylvain EngilbergeInstitut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, 71 avenue des Martyrs, CS 10090, 38044 Grenoble, France.ORCID https://orcid.org/0000-0001-8680-6790
Tristan WagnerMicrobial Protein Structure Group, Karl-von-Frisch-Strasse 10, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.ORCID https://orcid.org/0000-0002-3382-8969
Gianluca SantoniStructural Biology Group, European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38000 Grenoble, France.ORCID https://orcid.org/0000-0001-6215-998X
Cécile BreytonInstitut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, 71 avenue des Martyrs, CS 10090, 38044 Grenoble, France.ORCID https://orcid.org/0000-0002-4382-0434
Seigo ShimaMicrobial Protein Structure Group, Karl-von-Frisch-Strasse 10, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.ORCID https://orcid.org/0000-0003-1872-8705
Bruno FranzettiInstitut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, 71 avenue des Martyrs, CS 10090, 38044 Grenoble, France.ORCID https://orcid.org/0000-0001-5323-0510
Francois RiobéUniv. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie, F69342 Lyon, France.ORCID https://orcid.org/0000-0001-6746-8132
Olivier MauryUniv. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie, F69342 Lyon, France.ORCID https://orcid.org/0000-0002-4639-643X
Eric GirardInstitut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, 71 avenue des Martyrs, CS 10090, 38044 Grenoble, France.ORCID https://orcid.org/0000-0002-5758-6095
Centre National de la Recherche Scientifique · FRMax Planck Institute for Terrestrial Microbiology · DEUniversité Claude Bernard Lyon 1 · FREuropean Synchrotron Radiation Facility · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Obtaining crystals and solving the phase problem remain major hurdles encountered by bio-crystallographers in their race to obtain new high-quality structures. Both issues can be overcome by the crystallophore, Tb-Xo4, a lanthanide-based molecular complex with unique nucleating and phasing properties. This article presents examples of new crystallization conditions induced by the presence of Tb-Xo4. These new crystalline forms bypass crystal defects often encountered by crystallographers, such as low-resolution diffracting samples or crystals with twinning. Thanks to Tb-Xo4's high phasing power, the structure determination process is greatly facilitated and can be extended to serial crystallography approaches.

Indexed as

anomalous-scattering-based methodsde novo phasingmacromolecular crystallizationprotein crystallographyserial crystallographyTb-Xo4the crystallophore

Identifiers

PMID31396026
PMCPMC6662991
OpenAlexW2955584070

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.