ArticleJournal of applied crystallography2019
A temperature-controlled cold-gas humidifier and its application to protein crystals with the humid-air and glue-coating method.
Article in Journal of applied crystallography, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Macromolecular crystallography at SPring-8 and SACLA.Journal of synchrotron radiation · 2025Article
- Cryo2RT: a high-throughput method for room-temperature macromolecular crystallography from cryo-cooled crystals.Acta crystallographica. Section D, Structural biology · 2024Article
- Determining biomolecular structures near room temperature using X-ray crystallography: concepts, methods and future optimization.Acta crystallographica. Section D, Structural biology · 2023Article
- In situ crystal data-collection and ligand-screening system at SPring-8.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Evaluation of the data-collection strategy for room-temperature micro-crystallography studied by serial synchrotron rotation crystallography combined with the humid air and glue-coating method.Acta crystallographica. Section D, Structural biology · 2021Article
- Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals.Acta crystallographica. Section F, Structural biology communications · 2020Article
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7 authors.
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Abstract
The room-temperature experiment has been revisited for macromolecular crystallography. Despite being limited by radiation damage, such experiments reveal structural differences depending on temperature, and it is expected that they will be able to probe structures that are physiologically alive. For such experiments, the humid-air and glue-coating (HAG) method for humidity-controlled experiments is proposed. The HAG method improves the stability of most crystals in capillary-free experiments and is applicable at both cryogenic and ambient temperatures. To expand the thermal versatility of the HAG method, a new humidifier and a protein-crystal-handling workbench have been developed. The devices provide temperatures down to 4°C and successfully maintain growth at that temperature of bovine cytochrome c oxidase crystals, which are highly sensitive to temperature variation. Hence, the humidifier and protein-crystal-handling workbench have proved useful for temperature-sensitive samples and will help reveal temperature-dependent variations in protein structures.
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