ArticleScientific reports2019
Development of a viability digital PCR protocol for the selective detection and quantification of live Erwinia amylovora cells in cankers.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
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- Copper-induced stress mechanisms inApplied and environmental microbiology · 2025Article
- Development of a Droplet Digital PCR Assay Targeting the Internal Transcribed Spacer Gene for Rapid Detection ofPathogens (Basel, Switzerland) · 2025Article
- Improved Canker Processing and Viability Droplet Digital PCR Allow Detection ofMicroorganisms · 2024Article
- Single-Cell Detection ofSensors (Basel, Switzerland) · 2023Article
- Rapid Molecular Phenotypic Antimicrobial Susceptibility Test forACS infectious diseases · 2023Article
- Rapid molecular phenotypic antimicrobial susceptibility test forbioRxiv : the preprint server for biology · 2023Article
- Review
- Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future Outbreaks.Clinical microbiology reviews · 2022Review
- Recent Methods for the Viability Assessment of Bacterial Pathogens: Advances, Challenges, and Future Perspectives.Pathogens (Basel, Switzerland) · 2022Review
- qPCR screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture.Journal of fish diseases · 2022Article
- Host-derived chimeric peptides clear the causative bacteria and augment host innate immunity during infection: A case study of HLB in citrus and fire blight in apple.Frontiers in plant science · 2022Article
- Fire blight resistance, irrigation and conducive wet weather improveFrontiers in microbiology · 2022Article
- Improved quantitative microbiome profiling for environmental antibiotic resistance surveillance.Environmental microbiome · 2021Article
- Digital PCR: What Relevance to Plant Studies?Biology · 2020Review
- Moving from qPCR to Chip Digital PCR Assays for Tracking of someMicroorganisms · 2020Article
- Digital Absolute Gene Expression Analysis of Essential Starch-Related Genes in a Radiation DevelopedPlants (Basel, Switzerland) · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fire blight is a devastating disease of apple and pear caused by the bacterium Erwinia amylovora. One of its main symptoms is canker formation on perennial tissues which may lead to the death of limbs and/or the entire tree. E. amylovora overwinters in cankers which play an important role in initiating fire blight epidemics. However, knowledge of pathogen biology in cankers is scarce, in part due to limitations of classical microbiology methods and the inability of most molecular techniques to distinguish live from dead cells. In this work, a viability digital PCR (v-dPCR) protocol using propidium monoazide (PMA) was developed, allowing for the first time the selective detection and absolute quantification of E. amylovora live cells in apple and pear cankers collected in two time periods. Some key factors affecting the v-dPCR performance were the maceration buffer composition, the target DNA amplicon length, the thermal cycle number and the use of sodium dodecyl sulfate or PMA enhancer for Gram-negative bacteria to improve the effect of PMA. In the future, this methodology could shed light on E. amylovora population dynamics in cankers and provide clues on the effect of management practices, host cultivar, host water/nutritional status, etc., on bacterial survival.
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