ArticleMethods in cell biology2019
Quantifying autophagic flux in kidney tissue using structured illumination microscopy.
Kensei Taguchi, Bertha C Elias, Subo Qian, Craig R Brooks
Open access · greenAbstract read
In one paragraphArticle in Methods in cell biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
4 authors at 2 institutions in 1 country.
Kensei TaguchiDivision of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, United States.
Bertha C EliasDivision of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, United States.
Subo QianDivision of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, United States.
Craig R BrooksDivision of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, United States; Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, United States. Electronic address: craig.brooks@vumc.org.
Vanderbilt University Medical Center · USVanderbilt University · US
Funding
Tumor Immunology and Microenvironment Research ProgramP30CA068485 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI STACIE B DUSETZINA · 1995 to 2026
$172.8MTranslational Analysis CoreP30DK058404 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI RICHARD M. PEEK · 2002 to 2026
$29.9MVanderbilt Diabetes Research CenterP30DK020593 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI ALVIN C POWERS · 2012 to 2026
$29.3MShop Module CoreP30EY008126 · NEI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI David J. Calkins · 1989 to 2026
$19.6MVanderbilt Mouse Metabolic Physiology CenterU24DK059637 · NIDDK · VANDERBILT UNIVERSITY · PI WASSERMAN, DAVID H · 2001 to 2015
$14.9MVanderbilt O'Brien Kidney Center - Core D - Clinical and Translational CoreP30DK114809 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI DE CAESTECKER, MARK P. · 2017 to 2021
$5.9MThe role of cyclin G1 in chronic kidney diseaseR01DK121101 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Craig Robert Brooks · 2019 to 2026
$3.7MProximal tubule Kim-1 expression modulates acute and chronic kidney injuryK01DK099473 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI BROOKS, CRAIG ROBERT · 2013 to 2017
$787kNCI NIH HHS P30 CA068485NEI NIH HHS P30 EY008126NIDDK NIH HHS K01 DK099473NIDDK NIH HHS P30 DK020593NIDDK NIH HHS P30 DK058404NIDDK NIH HHS P30 DK114809NIDDK NIH HHS R01 DK121101NIDDK NIH HHS U24 DK059637
6 · The paper itselfAbstract
Kidney disease is estimated to affect 15% of the world's population. Autophagy is a key homeostatic pathway in eukaryotic cells, which has been linked to numerous pathological states. In the kidney, autophagy has been shown to modulate both acute and chronic injuries. Despite the importance of autophagy in kidney disease, few techniques to precisely monitor autophagic flux in kidney tissue are available. Here we describe an improved technique to quantify autophagic flux using an RFP-GFP-LC3 reporter mouse and super-resolution microscopy. Using structured illumination microscopy, we can resolve individual autophagosomes within kidney tubular cells. We describe the preparation of slides, staining, imaging and data processing. 3D surface rendering is utilized to categorize and quantify autophagosomes by number, size, fluorescence and autophagic flux in response to ischemia.
Indexed as
AnimalsAutophagosomesAutophagyDisease Models, AnimalEpithelial CellsGenes, ReporterGreen Fluorescent ProteinsHistocytological Preparation TechniquesHumansImaging, Three-DimensionalKidneyMiceMice, TransgenicMicroscopy, FluorescenceReperfusion InjuryGreen Fluorescent ProteinsAcute kidney injuryAutophagyStructured illumination microscopySuper-resolution microscopy
Identifiers
PMID31395381
PMCPMC10625164
OpenAlexW2955294900
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