ArticleEnvironmental health perspectives2019
Customized MethylC-Capture Sequencing to Evaluate Variation in the Human Sperm DNA Methylome Representative of Altered Folate Metabolism.
Article in Environmental health perspectives, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Association of the Serum Folate and Total Calcium and Magnesium Levels Before Ovarian Stimulation With Outcomes of FreshFrontiers in endocrinology · 2022Trial
- Altered sperm DNA methylation provides an epigenetic route from overweight and older men to intergenerational disease transmission.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Association between serum vitamins and sex hormone levels in adolescent males: based on the National Health and Nutrition Examination Survey (NHANES).Hormones (Athens, Greece) · 2026Article
- Type 2 diabetes impacts DNA methylation in human sperm.Clinical epigenetics · 2025Article
- Exploring the Epigenetic Landscape of Spermatozoa: Impact of Oxidative Stress and Antioxidant Supplementation on DNA Methylation and Hydroxymethylation.Antioxidants (Basel, Switzerland) · 2024Article
- Sperm DNA methylation defects in a new mouse model of the 5,10-methylenetetrahydrofolate reductase 677C>T variant and correction with moderate dose folic acid supplementation.Molecular human reproduction · 2024Article
- Characterization of genomic regions escaping epigenetic reprogramming in sheep.Environmental epigenetics · 2024Article
- The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men.Environmental health perspectives · 2024Article
- Higher incidence of embryonic defects in mouse offspring conceived with assisted reproduction from fathers with sperm epimutations.Human molecular genetics · 2023Article
- Capturing sex-specific and hypofertility-linked effects of assisted reproductive technologies on the cord blood DNA methylome.Clinical epigenetics · 2023Article
- Emerging evidence that the mammalian sperm epigenome serves as a template for embryo development.Nature communications · 2023Review
- Sperm DNA methylome abnormalities occur both pre- and post-treatment in men with Hodgkin disease and testicular cancer.Clinical epigenetics · 2023Article
- Altered DNA methylation in estrogen-responsive repetitive sequences of spermatozoa of infertile men with shortened anogenital distance.Clinical epigenetics · 2022Article
- Exposure to environmental contaminants and folic acid supplementation intergenerationally impact fetal skeleton development through the paternal lineage in a rat model.Frontiers in toxicology · 2022Article
- Evaluation of acute myeloid leukemia blast percentage on MethylC-Capture Sequencing results.Experimental hematology & oncology · 2021Article
- DNA Methylation Differences Between Zona Pellucida-Bound and Manually Selected Spermatozoa Are Associated With Autism Susceptibility.Frontiers in endocrinology · 2021Article
- High-resolution analyses of human sperm dynamic methylome reveal thousands of novel age-related epigenetic alterations.Clinical epigenetics · 2020Article
- Capturing functional epigenomes for insight into metabolic diseases.Molecular metabolism · 2020Review
Corrections and comments
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Authors and funding
17 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe sperm DNA methylation landscape is unique and critical for offspring health. If gamete-derived DNA methylation escapes reprograming in early embryos, epigenetic defects in sperm may be transmitted to the next generation. Current techniques to assess sperm DNA methylation show bias toward CpG-dense regions and do not target areas of dynamic methylation, those predicted to be environmentally sensitive and tunable regulatory elements.
objectivesOur goal was to assess variation in human sperm DNA methylation and design a targeted capture panel to interrogate the human sperm methylome.
methodsTo characterize variation in sperm DNA methylation, we performed whole genome bisulfite sequencing (WGBS) on an equimolar pool of sperm DNA from a wide cross section of 30 men varying in age, fertility status, methylenetetrahydrofolate reductase (
resultsThrough WGBS we discovered nearly 1 million CpGs possessing intermediate methylation levels (20-80%), termed dynamic sperm CpGs. These dynamic CpGs, along with 2 million commonly assessed CpGs, were used to customize a capture panel for targeted interrogation of the human sperm methylome and test its ability to detect effects of altered folate metabolism. As compared with DISCUSSION: Our sperm panel allowed the discovery of differential methylation following conditions affecting folate metabolism in novel dynamic sperm CpGs. Improved ability to examine variation in sperm DNA methylation can facilitate comprehensive studies of environment-epigenome interactions. https://doi.org/10.1289/EHP4812.
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