ArticleNucleic acids research2019
AGO-unbound cytosolic pool of mature miRNAs in plant cells reveals a novel regulatory step at AGO1 loading.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
- Tissue specific RISC-loading reassesses small RNA functionality in developing pepper fruit.Plant molecular biology · 2026Article
- Cross-kingdom RNAi: a universal mechanism of inter-organismal communication with many unknowns.Journal of experimental botany · 2026Review
- Plant microRNA maturation and function.Nature reviews. Molecular cell biology · 2026Review
- Detection of MicroRNAs Released from Argonautes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The nuclear pore complex acts as a hub for pri-miRNA transcription and processing in plants.Nucleic acids research · 2025Article
- Remote precursor elements can modulate RNA induced silencing complex-loading efficiency of miR168 in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- RH3 enhances antiviral defense by facilitating small RNA loading into Argonaute 2 at endoplasmic reticulum-chloroplast membrane contact sites.Nature communications · 2025Article
- Comparative functional analyses of the movement and coat proteins of grapevine Pinot gris virus, encoded by symptomatic and asymptomatic variants.Frontiers in plant science · 2025Article
- Review
- miR-7 is recruited to the high molecular weight RNA-induced silencing complex in CD8RNA (New York, N.Y.) · 2023Article
- Beyond Loading: Functions of Plant ARGONAUTE Proteins.International journal of molecular sciences · 2023Review
- Recent Insights into Plant miRNA Biogenesis: Multiple Layers of miRNA Level Regulation.Plants (Basel, Switzerland) · 2023Review
- microRNA production inFrontiers in plant science · 2023Review
- NODULIN HOMEOBOX is required for heterochromatin homeostasis in Arabidopsis.Nature communications · 2022Article
- Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis.Developmental cell · 2022Article
- Arabidopsis RBV is a conserved WD40 repeat protein that promotes microRNA biogenesis and ARGONAUTE1 loading.Nature communications · 2022Article
- Plant and animal small RNA communications between cells and organisms.Nature reviews. Molecular cell biology · 2022Review
- Ecotype-specific blockage of tasiARF production by two different RNA viruses in Arabidopsis.PloS one · 2022Article
- A microRNA-microRNA crosstalk network inferred from genome-wide single nucleotide polymorphism variants in natural populations ofFrontiers in plant science · 2022Article
- InFrontiers in plant science · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) is mediated by small, 20-24-nt-long, non-coding regulatory (s)RNAs such as micro (mi) and small interfering (si) RNAs via the action of ARGONAUTE (AGO) proteins. High-throughput sequencing of size-separated sRNA pools of plant crude extracts revealed that the majority of the canonical miRNAs were associated with high molecular weight RNA-induced silencing complexes co-migrating with AGO1 (HMW RISC). In contrast, the majority of 24-nt-long siRNAs were found in association with low molecular weight complexes co-migrating with AGO4 (LMW RISC). Intriguingly, we identified a large set of cytoplasmic sRNAs, including mature miRNA sequences, in the low molecular size range corresponding to protein-unbound sRNAs. By comparing the RISC-loaded and protein-unbound pools of miRNAs, we identified miRNAs with highly different loading efficiencies. Expression of selected miRNAs in transient and transgenic systems validated their altered loading abilities implying that this process is controlled by information associated with the diverse miRNA precursors. We also showed that the availability of AGO proteins is a limiting factor determining the loading efficiency of miRNAs. Our data reveal the existence of a regulatory checkpoint determining the RISC-loading efficiencies of various miRNAs by sorting only a subset of the produced miRNAs into the biologically active RISCs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.