ArticleeLife2019
Temporal regulation of axonal repulsion by alternative splicing of a conserved microexon in mammalian
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Conservation of extended sequence and structure in the branchpoint-to-3' splice site region upstream of neural microexons.bioRxiv : the preprint server for biology · 2026Article
- miR-219a-Robo1 axis acts as a conserved switch for slit-mediated commissural axon guidance.iScience · 2025Article
- The Slit-Robo signalling pathway in nervous system development: a comparative perspective from vertebrates and invertebrates.Open biology · 2025Review
- Regulated microexon alternative splicing in single neurons tunes synaptic function.EMBO reports · 2025Article
- Loss ofCurrent issues in molecular biology · 2025Article
- Article
- A cell-type-specific alternative splicing regulator shapes synapse properties in a trans-synaptic manner.Cell reports · 2023Article
- The Musashi proteins direct post-transcriptional control of protein expression and alternate exon splicing in vertebrate photoreceptors.Communications biology · 2022Article
- RBFOX2-regulated TEAD1 alternative splicing plays a pivotal role in Hippo-YAP signaling.Nucleic acids research · 2022Article
- Multilayered regulations of alternative splicing, NMD, and protein stability control temporal induction and tissue-specific expression of TRIM46 during axon formation.Nature communications · 2022Article
- Spliceosomal component PRP-40 is a central regulator of microexon splicing.Cell reports · 2021Article
- RNA processing in neurological tissue: development, aging and disease.Seminars in cell & developmental biology · 2021Review
- Crosstalk between Bone and Nerves within Bone.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- MicroExonator enables systematic discovery and quantification of microexons across mouse embryonic development.Genome biology · 2021Article
- Dorsal commissural axon guidance in the developing spinal cord.Current topics in developmental biology · 2021Review
- The Fine Art of Writing a Message: RNA Metabolism in the Shaping and Remodeling of the Nervous System.Frontiers in molecular neuroscience · 2021Review
- Robo2 Receptor Gates the Anatomical Divergence of Neurons Derived From a Common Precursor Origin.Frontiers in cell and developmental biology · 2021Article
- New insights into the molecular mechanisms of axon guidance receptor regulation and signaling.Current topics in developmental biology · 2021Review
- Molecular mechanisms regulating axon responsiveness at the midline.Developmental biology · 2020Article
- Alternative splicing programming of axon formation.Wiley interdisciplinary reviews. RNA · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Proper connectivity of the nervous system requires temporal and spatial control of axon guidance signaling. As commissural axons navigate across the CNS midline, ROBO-mediated repulsion has traditionally been thought to be repressed before crossing, and then to become upregulated after crossing. The regulation of the ROBO receptors involves multiple mechanisms that control protein expression, trafficking, and activity. Here, we report that mammalian ROBO1 and ROBO2 are not uniformly inhibited precrossing and are instead subject to additional temporal control via alternative splicing at a conserved microexon. The NOVA splicing factors regulate the developmental expression of ROBO1 and ROBO2 variants with small sequence differences and distinct guidance activities. As a result, ROBO-mediated axonal repulsion is activated early in development to prevent premature crossing and becomes inhibited later to allow crossing. Postcrossing, the ROBO1 and ROBO2 isoforms are disinhibited to prevent midline reentry and to guide postcrossing commissural axons to distinct mediolateral positions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.