ArticleStem cells and development2019
Investigation of Postnatal Craniofacial Bone Development with Tissue Clearing-Based Three-Dimensional Imaging.
Article in Stem cells and development, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Targeting Gli1Stem cell reviews and reports · 2026Review
- Hedgehog signaling pathway is an influential factor on vascular biology: a review.Journal of physiology and biochemistry · 2025Review
- Palatal segment contributions to midfacial anterior-posterior growth.Journal of anatomy · 2025Article
- Crosstalk Between Wnt/β-Catenin and Hedgehog Supports Gli1+ Lineage Osteogenesis in Cranial Sutures.International journal of molecular sciences · 2025Article
- Cyclic loading failed to promote growth in a pig model of midfacial hypoplasia.Journal of anatomy · 2024Article
- Palatal segment contributions to midfacial anterior-posterior growth.bioRxiv : the preprint server for biology · 2024Article
- The Role of Gli1Biomolecules · 2023Review
- Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development.Science advances · 2023Article
- Three-Dimensional Imaging and Quantitative Analysis of Blood Vessel Distribution in The Meniscus of Transgenic Mouse after Tissue Clearing.Cell journal · 2023Article
- Review
- Age-related transversal changes in craniofacial sutures of the anterior viscerocranium in growing rats.Frontiers in physiology · 2023Article
- State-of-the-art techniques for imaging the vascular microenvironment in craniofacial bone tissue engineering applications.American journal of physiology. Cell physiology · 2022Review
- Quantitative 3D imaging of the cranial microvascular environment at single-cell resolution.Nature communications · 2021Article
- Recent advances in stem cell therapy for neurodegenerative disease: Three dimensional tracing and its emerging use.World journal of stem cells · 2021Review
- Cranial Suture Mesenchymal Stem Cells: Insights and Advances.Biomolecules · 2021Review
- Tissue clearing technique: Recent progress and biomedical applications.Journal of anatomy · 2021Review
- The Intertwined Evolution and Development of Sutures and Cranial Morphology.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Traditional two-dimensional histological sections and microcomputed tomography remain to be the major tools for studying craniofacial bones despite the complicated spatial organization of craniofacial organs. Recently, our laboratory developed the Poly(Ethylene Glycol) Associated Solvent System (PEGASOS) tissue clearing method, which can efficiently render hard tissues, including bones and teeth fully transparent without losing endogenous fluorescent signals. Complete tissue transparency enables us to acquire three-dimensional (3D) images of craniofacial bone vasculature, osteogenesis utilizing various labeling strategies, thus to investigate the spatial relationship among different tissues during postnatal craniofacial development. We found out that during the early stage of postnatal development, craniofacial osteogenesis occurs throughout the entire craniofacial bones, including the periosteum, dura, bone marrow, and suture. After 3-4 weeks, craniofacial osteogenesis is gradually restricted to the suture region and remaining bone marrow space. Similarly, craniofacial bone vasculature gradually restricts to the suture region. Osteogenesis is spatially associated with vasculature during the entire postnatal development. Importantly, we demonstrated that in adult calvarial bones, Gli1+ mesenchymal stem cells were also spatially associated with the vasculature. These findings indicate that craniofacial bones share similar osteogenesis mechanism as the long bone despite their distinct osteogenic mechanisms. In addition, the PEGASOS tissue clearing method-based 3D imaging technique is a useful new tool for craniofacial research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.