Evidence map›Paper›PMID 31392501›Full record

ArticleCurrent microbiology2019

The gal80 Deletion by CRISPR-Cas9 in Engineered Saccharomyces cerevisiae Produces Artemisinic Acid Without Galactose Induction.

Limei Ai, Weiwei Guo, Wei Chen, Yun Teng, Liping Bai

Abstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. ACS synthetic biology · 2024
    Article
  2. Review
  3. EngineeredJournal of fungi (Basel, Switzerland) · 2022
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Limei AiNHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tian Tan Xi Li No. 1, Beijing, 100050, China.
Weiwei GuoNHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tian Tan Xi Li No. 1, Beijing, 100050, China.
Wei ChenZhejiang Key Laboratory of Antifungal Drugs, Zhejiang Hisun Pharmaceutical Company Limited, Taizhou, 318000, Zhejiang, China.
Yun TengZhejiang Key Laboratory of Antifungal Drugs, Zhejiang Hisun Pharmaceutical Company Limited, Taizhou, 318000, Zhejiang, China. yteng@hisunpharm.com.
Liping BaiNHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tian Tan Xi Li No. 1, Beijing, 100050, China. lipingbai1973@163.com.ORCID http://orcid.org/0000-0002-5179-5005
Chinese Academy of Medical Sciences & Peking Union Medical College · CNZhejiang Hisun Pharmaceutical (China) · CN

Funding

Drug Innovation Major Project 2018ZX09711001-007-003National Key Research and Development Program of China 2018YFA0902000National Natural Science Foundation of China 31870059The Drug Innovation Major Project 2017ZX09101002-003-003
6 · The paper itself

Abstract

The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas system has emerged as the dominating tool for genome engineering, while also changes the speed and efficiency of metabolic engineering in conventional and non-conventional yeasts. Among these CRISPR-Cas systems, CRISPR-Cas9 technology has usually been applied for removing unfavorable target genes. Here, we used CRISPR-Cas9 technology to delete the gal80 gene in uracil-deficient strain and had successfully remolded the engineered Saccharomyces cerevisiae that can produce artemisinic acid without galactose induction. An L

Indexed as

ArtemisininsCRISPR-Cas SystemsFermentationGalactoseGene DeletionMetabolic EngineeringRepressor ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsUracilartemisic acidArtemisininsGAL80 protein, S cerevisiaeGalactoseRepressor ProteinsSaccharomyces cerevisiae ProteinsUracil

Identifiers

PMID31392501
OpenAlexW2966798017

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.